Photomanipulation of Minimal Synthetic Cells: Area Increase, Softening, and Interleaflet Coupling of Membrane Models Doped with Azobenzene-Lipid Photoswitches.
atomic force microscopy (AFM)
azo-PC
bending rigidity
giant vesicles
membrane capacitance
molecular dynamics simulations
photoswitchable lipids
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
11 2023
11 2023
Historique:
revised:
24
07
2023
received:
28
06
2023
medline:
6
11
2023
pubmed:
1
9
2023
entrez:
1
9
2023
Statut:
ppublish
Résumé
Light can effectively interrogate biological systems in a reversible and physiologically compatible manner with high spatiotemporal precision. Understanding the biophysics of photo-induced processes in bio-systems is crucial for achieving relevant clinical applications. Employing membranes doped with the photolipid azobenzene-phosphatidylcholine (azo-PC), a holistic picture of light-triggered changes in membrane kinetics, morphology, and material properties obtained from correlative studies on cell-sized vesicles, Langmuir monolayers, supported lipid bilayers, and molecular dynamics simulations is provided. Light-induced membrane area increases as high as ≈25% and a ten-fold decrease in the membrane bending rigidity is observed upon trans-to-cis azo-PC isomerization associated with membrane leaflet coupling and molecular curvature changes. Vesicle electrodeformation measurements and atomic force microscopy reveal that trans azo-PC bilayers are thicker than palmitoyl-oleoyl phosphatidylcholine (POPC) bilayers but have higher specific membrane capacitance and dielectric constant suggesting an increased ability to store electric charges across the membrane. Lastly, incubating POPC vesicles with azo-PC solutions results in the insertion of azo-PC in the membrane enabling them to become photoresponsive. All these results demonstrate that light can be used to finely manipulate the shape, mechanical and electric properties of photolipid-doped minimal cell models, and liposomal drug carriers, thus, presenting a promising therapeutic alternative for the repair of cellular disorders.
Identifiants
pubmed: 37653602
doi: 10.1002/advs.202304336
pmc: PMC10625111
doi:
Substances chimiques
azobenzene
F0U1H6UG5C
Phosphatidylcholines
0
Liposomes
0
Lipid Bilayers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2304336Subventions
Organisme : International Max Planck Research School
Organisme : Germany's Excellence Strategy
ID : EXC 2008/1
Organisme : Germany's Excellence Strategy
ID : 390540038
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
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