Light-Induced Orthogonal Fragmentation of Crosslinked Peptides.
Journal
JACS Au
ISSN: 2691-3704
Titre abrégé: JACS Au
Pays: United States
ID NLM: 101775714
Informations de publication
Date de publication:
28 Aug 2023
28 Aug 2023
Historique:
received:
19
04
2023
revised:
02
08
2023
accepted:
03
08
2023
medline:
1
9
2023
pubmed:
1
9
2023
entrez:
1
9
2023
Statut:
epublish
Résumé
Crosslinking mass spectrometry provides pivotal information on the structure and interaction of proteins. MS-cleavable crosslinkers are regarded as a cornerstone for the analysis of complex mixtures. Yet they fragment under similar conditions as peptides, leading to mixed fragmentation spectra of the crosslinker and peptide. This hampers selecting individual peptides for their independent identification. Here, we introduce orthogonal cleavage using ultraviolet photodissociation (UVPD) to increase crosslinker over peptide fragmentation. We designed and synthesized a crosslinker that can be cleaved at 213 nm in a commercial mass spectrometer configuration. In an analysis of crosslinked
Identifiants
pubmed: 37654600
doi: 10.1021/jacsau.3c00199
pmc: PMC10466327
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2123-2130Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© 2023 The Authors. Published by American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
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