Dexmedetomidine Promoted HSPB8 Expression via Inhibiting the lncRNA SNHG14/UPF1 Axis to Inhibit Apoptosis of Nerve Cells in AD : The Role of Dexmedetomidine in AD.
Alzheimer’s disease
Dexmedetomidine
HSPB8
UPF1
lncRNA SNHG14
Journal
Neurotoxicity research
ISSN: 1476-3524
Titre abrégé: Neurotox Res
Pays: United States
ID NLM: 100929017
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
14
09
2022
accepted:
26
05
2023
revised:
16
05
2023
medline:
14
9
2023
pubmed:
1
9
2023
entrez:
1
9
2023
Statut:
ppublish
Résumé
Dexmedetomidine (Dex) is reported to play a neuroprotective role in Alzheimer's disease (AD). However, the specific mechanism remains unclear. Figure out the underlying molecular mechanism of Dex regulating nerve cell apoptosis in the AD model. The AD model in vitro was established after SH-SY5Y cells were treated with Aβ1 - 42 at (10 μM) for 24 h. The interaction among UPF1, lncRNA SNHG14, and HSPB8 was verified by RIP assay. Cell viability, apoptosis, the level of genes, and proteins were detected by CCK-8 assay, flow cytometry, Western blot, and qRT-PCR, respectively. Dex downregulated lncRNA SNHG14 level and inhibited apoptosis of nerve cells. LncRNA SNHG14 overexpression reversed the inhibitory effect of Dex on nerve cell apoptosis in the AD model. LncRNA SNHG14 attenuated HSPB8 mRNA stability by recruiting UPF1. HSPB8 overexpression inhibited apoptosis of nerve cells in the AD model. Moreover, HSPB8 knockdown reversed the inhibitory effect of Dex on nerve cell apoptosis in the AD model. Our study demonstrated that Dex promoted HSPB8 expression via inhibiting the lncRNA SNHG14/UPF1 axis to inhibit nerve cell apoptosis in AD.
Identifiants
pubmed: 37656385
doi: 10.1007/s12640-023-00653-4
pii: 10.1007/s12640-023-00653-4
doi:
Substances chimiques
RNA, Long Noncoding
0
Dexmedetomidine
67VB76HONO
Molecular Chaperones
0
UPF1 protein, human
EC 3.6.4.13
Trans-Activators
0
RNA Helicases
EC 3.6.4.13
HSPB8 protein, human
0
Heat-Shock Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
471-480Subventions
Organisme : the Scientific Research Project of Heilongjiang Health Commission
ID : 2019-314
Organisme : the Scientific Research Project of Heilongjiang Health Commission
ID : 20210404110036
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Banesh S, Trivedi V (2021) CD36 ectodomain detects apoptosis in mammalian cells. Mol Biotechnol 63(11):992–1003
doi: 10.1007/s12033-021-00356-1
pubmed: 34173181
Chen Y, Peng C, Chen J, Chen D, Yang B, He B et al (2019) WTAP facilitates progression of hepatocellular carcinoma via m6A-HuR-dependent epigenetic silencing of ETS1. Mol Cancer 18(1):127
doi: 10.1186/s12943-019-1053-8
pubmed: 31438961
pmcid: 6704583
Ding XD, Cao YY, Li L, Zhao GY (2021) Dexmedetomidine reduces the lidocaine-induced neurotoxicity by inhibiting inflammasome activation and reducing pyroptosis in rats. Biol Pharm Bull 44(7):902–909
doi: 10.1248/bpb.b20-00482
pubmed: 34193687
Dong H, Wang W, Chen R, Zhang Y, Zou K, Ye M et al (2018) Exosome-mediated transfer of lncRNA-SNHG14 promotes trastuzumab chemoresistance in breast cancer. Int J Oncol 53(3):1013–1026
pubmed: 30015837
pmcid: 6065402
Dong H, Wang W, Mo S, Liu Q, Chen X, Chen R et al (2018) Long non-coding RNA SNHG14 induces trastuzumab resistance of breast cancer via regulating PABPC1 expression through H3K27 acetylation. J Cell Mol Med 22(10):4935–4947
doi: 10.1111/jcmm.13758
pubmed: 30063126
pmcid: 6156344
Duan R, Wang SY, Wei B, Deng Y, Fu XX, Gong PY et al (2021) Angiotensin-(1–7) analogue AVE0991 modulates astrocyte-mediated neuroinflammation via lncRNA SNHG14/miR-223-3p/NLRP3 pathway and offers neuroprotection in a transgenic mouse model of Alzheimer’s disease. J Inflamm Res 14:7007–7019
doi: 10.2147/JIR.S343575
pubmed: 34955647
pmcid: 8694579
Faghihi MA, Modarresi F, Khalil AM, Wood DE, Sahagan BG, Morgan TE et al (2008) Expression of a noncoding RNA is elevated in Alzheimer’s disease and drives rapid feed-forward regulation of beta-secretase. Nat Med 14(7):723–730
doi: 10.1038/nm1784
pubmed: 18587408
pmcid: 2826895
Fotuhi SN, Khalaj-Kondori M, HoseinpourFeizi MA, Talebi M (2019) Long non-coding RNA BACE1-AS may serve as an Alzheimer’s disease blood-based biomarker. J Mol Neurosci MN 69(3):351–359
doi: 10.1007/s12031-019-01364-2
pubmed: 31264051
Han S, Cao D, Sha J, Zhu X, Chen D (2020) LncRNA ZFPM2-AS1 promotes lung adenocarcinoma progression by interacting with UPF1 to destabilize ZFPM2. Mol Oncol 14(5):1074–1088
doi: 10.1002/1878-0261.12631
pubmed: 31919993
pmcid: 7191191
He Y, Qiang Y (2021) Mechanism of autonomic exercise improving cognitive function of Alzheimer’s disease by regulating lncRNA SNHG14. Am J Alzheimers Dis Other Demen 36:15333175211027680
doi: 10.1177/15333175211027681
pubmed: 34338033
Hong H, Mo Y, Li D, Xu Z, Liao Y, Yin P et al (2020) Aberrant expression profiles of lncRNAs and their associated nearby coding genes in the hippocampus of the SAMP8 mouse model with AD. Molec Ther Nucleic acids 20:140–54
Hu G, Shi Z, Shao W, Xu B (2022) MicroRNA-214-5p involves in the protection effect of dexmedetomidine against neurological injury in Alzheimer’s disease via targeting the suppressor of zest 12. Brain Res Bull 178:164–172
doi: 10.1016/j.brainresbull.2021.10.016
pubmed: 34715270
Huaying C, Xing J, Luya J, Linhui N, Di S, Xianjun D (2020) A signature of five long non-coding RNAs for predicting the prognosis of Alzheimer’s disease based on competing endogenous RNA networks. Front Aging Neurosci 12:598606
doi: 10.3389/fnagi.2020.598606
pubmed: 33584243
Jiang H, Ni J, Zheng Y, Xu Y (2021) Knockdown of lncRNA SNHG14 alleviates LPS-induced inflammation and apoptosis of PC12 cells by regulating miR-181b-5p. Exp Ther Med 21(5):497
doi: 10.3892/etm.2021.9928
pubmed: 33791006
pmcid: 8005701
Lashley T, Schott JM, Weston P, Murray CE, Wellington H, Keshavan A et al (2018) Molecular biomarkers of Alzheimer’s disease: progress and prospects. Dis Mod Mech 11(5)
Li D, Zhang J, Li X, Chen Y, Yu F, Liu Q (2021) Insights into lncRNAs in Alzheimer’s disease mechanisms. RNA Biol 18(7):1037–1047
doi: 10.1080/15476286.2020.1788848
pubmed: 32605500
Li H, Zhang X, Chen M, Chen J, Gao T, Yao S (2018) Dexmedetomidine inhibits inflammation in microglia cells under stimulation of LPS and ATP by c-Fos/NLRP3/caspase-1 cascades. EXCLI J 17:302–311
pubmed: 29743866
pmcid: 5938529
Li Z, Ho IHT, Li X, Xu D, Wu WKK, Chan MTV et al (2019) Long non-coding RNAs in the spinal cord injury: novel spotlight. J Cell Mol Med 23(8):4883–4890
doi: 10.1111/jcmm.14422
pubmed: 31140726
pmcid: 6653026
Lou CH, Shao A, Shum EY, Espinoza JL, Huang L, Karam R et al (2014) Posttranscriptional control of the stem cell and neurogenic programs by the nonsense-mediated RNA decay pathway. Cell Rep 6(4):748–764
doi: 10.1016/j.celrep.2014.01.028
pubmed: 24529710
pmcid: 3962089
Lu J, Liu X, Zheng J, Song J, Liu Y, Ruan X et al (2020) Lin28A promotes IRF6-regulated aerobic glycolysis in glioma cells by stabilizing SNHG14. Cell Death Dis 11(6):447
doi: 10.1038/s41419-020-2650-6
pubmed: 32527996
pmcid: 7289837
Pei CL, Fei KL, Yuan XY, Gong XJ (2019) LncRNA DANCR aggravates the progression of ovarian cancer by downregulating UPF1. Eur Rev Med Pharmacol Sci 23(24):10657–10663
pubmed: 31858532
Qin H, Ni H, Liu Y, Yuan Y, Xi T, Li X et al (2020) RNA-binding proteins in tumor progression. J Hematol Oncol 13(1):90
doi: 10.1186/s13045-020-00927-w
pubmed: 32653017
pmcid: 7353687
Ren Q, Zhao S, Ren C, Ma Z (2018) Astragalus polysaccharide alleviates LPS-induced inflammation injury by regulating miR-127 in H9c2 cardiomyoblasts. Int J Immunopathol Pharmacol 32:2058738418759180
pubmed: 29451405
Riva P, Ratti A, Venturin M (2016) The long non-coding RNAs in neurodegenerative diseases: novel mechanisms of pathogenesis. Curr Alzheimer Res 13(11):1219–1231
doi: 10.2174/1567205013666160622112234
pubmed: 27338628
Rusmini P, Cortese K, Crippa V, Cristofani R, Cicardi ME, Ferrari V et al (2019) Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration. Autophagy 15(4):631–651
doi: 10.1080/15548627.2018.1535292
pubmed: 30335591
Sun W, Zhao J, Li C (2020) Dexmedetomidine provides protection against hippocampal neuron apoptosis and cognitive impairment in mice with Alzheimer’s disease by mediating the miR-129/YAP1/JAG1 axis. Mol Neurobiol 57(12):5044–5055
doi: 10.1007/s12035-020-02069-z
pubmed: 32839917
Sun YB, Zhao H, Mu DL, Zhang W, Cui J, Wu L et al (2019) Dexmedetomidine inhibits astrocyte pyroptosis and subsequently protects the brain in in vitro and in vivo models of sepsis. Cell Death Dis 10(3):167
doi: 10.1038/s41419-019-1416-5
pubmed: 30778043
pmcid: 6379430
Ta Na HS, An M, Zhang T, Deni W, Hou L, Jin K (2022) Dexmedetomidine inhibits microglial activation through SNHG14/HMGB1 pathway in spinal cord ischemia-reperfusion injury mice. Int J Neurosci 132(1):77–88
doi: 10.1080/00207454.2020.1835901
pubmed: 33045891
Vendredy L, Adriaenssens E, Timmerman V (2020) Small heat shock proteins in neurodegenerative diseases. Cell Stress Chaperones 25(4):679–699
doi: 10.1007/s12192-020-01101-4
pubmed: 32323160
pmcid: 7332613
Vicario M, Skaper SD, Negro A (2014) The small heat shock protein HspB8: role in nervous system physiology and pathology. CNS Neurol Disord: Drug Targets 13(5):885–895
doi: 10.2174/1871527313666140711093344
pubmed: 25012617
Wang K, Wu M, Xu J, Wu C, Zhang B, Wang G et al (2019) Effects of dexmedetomidine on perioperative stress, inflammation, and immune function: systematic review and meta-analysis. Br J Anaesth 123(6):777–794
doi: 10.1016/j.bja.2019.07.027
pubmed: 31668347
Wang X, Lai Q, He J, Li Q, Ding J, Lan Z et al (2019) LncRNA SNHG6 promotes proliferation, invasion and migration in colorectal cancer cells by activating TGF-β/Smad signaling pathway via targeting UPF1 and inducing EMT via regulation of ZEB1. Int J Med Sci 16(1):51–59
doi: 10.7150/ijms.27359
pubmed: 30662328
pmcid: 6332483
Wang X, Yu X, Wei W, Liu Y (2020) Long noncoding RNA MACC1-AS1 promotes the stemness of nonsmall cell lung cancer cells through promoting UPF1-mediated destabilization of LATS1/2. Environ Toxicol 35(9):998–1006
doi: 10.1002/tox.22936
pubmed: 32401390
Wilhelmus MM, Boelens WC, Otte-Höller I, Kamps B, Kusters B, Maat-Schieman ML et al (2006) Small heat shock protein HspB8: its distribution in Alzheimer’s disease brains and its inhibition of amyloid-beta protein aggregation and cerebrovascular amyloid-beta toxicity. Acta Neuropathol 111(2):139–149
doi: 10.1007/s00401-005-0030-z
pubmed: 16485107
Xie X, Lin J, Liu J, Huang M, Zhong Y, Liang B et al (2019) A novel lncRNA NR4A1AS up-regulates orphan nuclear receptor NR4A1 expression by blocking UPF1-mediated mRNA destabilization in colorectal cancer. Clin Sci (London, England : 1979) 133(13):1457–73
Xue F, Zhang W, Chu HC (2016) Assessing perioperative dexmedetomidine reduces the incidence and severity of acute kidney injury following valvular heart surgery. Kidney Int 89(5):1164
doi: 10.1016/j.kint.2015.12.053
pubmed: 27083295
Yuan J, Wu Y, Li L, Liu C (2020) MicroRNA-425–5p promotes tau phosphorylation and cell apoptosis in Alzheimer’s disease by targeting heat shock protein B8. J Neural Transm (Vienna, Austria : 1996) 127(3):339–46
Zaepfel BL, Zhang Z, Maulding K, Coyne AN, Cheng W, Hayes LR et al (2021) UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction. Cell Rep 34(13):108925
doi: 10.1016/j.celrep.2021.108925
pubmed: 33789100
pmcid: 8063722
Zhang LM, Wang MH, Yang HC, Tian T, Sun GF, Ji YF et al (2019) Dopaminergic neuron injury in Parkinson’s disease is mitigated by interfering lncRNA SNHG14 expression to regulate the miR-133b/ α-synuclein pathway. Aging 11(21):9264–9279
doi: 10.18632/aging.102330
pubmed: 31683259
pmcid: 6874444
Zhong Y, Yu C, Qin W (2019) LncRNA SNHG14 promotes inflammatory response induced by cerebral ischemia/reperfusion injury through regulating miR-136-5p /ROCK1. Cancer Gene Ther 26(7–8):234–247
doi: 10.1038/s41417-018-0067-5
pubmed: 30546117