Applications of Environmental DNA (eDNA) in Monitoring the Endangered Status and Evaluating the Stock Enhancement Effect of Tropical Sea Cucumber Holothuria Scabra.
Endangered species
Environmental DNA
Resource assessment
Sea cucumber
TaqMan
Journal
Marine biotechnology (New York, N.Y.)
ISSN: 1436-2236
Titre abrégé: Mar Biotechnol (NY)
Pays: United States
ID NLM: 100892712
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
05
06
2023
accepted:
01
08
2023
medline:
23
11
2023
pubmed:
2
9
2023
entrez:
1
9
2023
Statut:
ppublish
Résumé
The tropical sea cucumber Holothuria scabra is naturally found in the Indo-West Pacific. However, due to their commercial value, natural H. scabra populations have declined significantly in recent years, resulting in its status as an endangered species. Surveys of H. scabra resource pose a challenge due to its specific characteristics, such as sand-burrowing behavior. To overcome this problem, our study established a convenient and feasible method for assessing H. scabra resources using environmental DNA (eDNA) monitoring technology. First, H. scabra-specific TaqMan primers and probe were designed based on its cox1 gene, followed by the development of an eDNA monitoring method for H. scabra in two separate sea areas (Xuwen and Daya Bay). The method was subsequently employed to investigate the distribution of H. scabra and assess the effects of aquaculture stock enhancement through juvenile releasing in the Weizhou Island sea area. The H. scabra eDNA monitoring approach was found to be more appropriate and credible than traditional methods, and a positive impact of stocking on H. scabra populations was observed. In summary, this is the first report to quantify eDNA concentration in a Holothuroidea species, and it provides a convenient and accurate method for surveying H. scabra resources. This study introduces novel concepts for eDNA-based detection of endangered marine benthic animals and monitoring their population distribution and abundance.
Identifiants
pubmed: 37658250
doi: 10.1007/s10126-023-10239-y
pii: 10.1007/s10126-023-10239-y
doi:
Substances chimiques
DNA, Environmental
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
778-789Subventions
Organisme : National Key Research and Development Program of China
ID : 2020YFD0901104
Organisme : National Key Research and Development Program of China
ID : 2018YFD0901605
Organisme : National Key Research and Development Program of China
ID : 2022YFD2401301
Organisme : Scientific Research and Technology Development Program of Guangxi
ID : AD22035965
Organisme : National Natural Science Foundation of China
ID : 41906101
Organisme : National Natural Science Foundation of China
ID : 42176132
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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