Low levels of mouse sperm chromatin fragmentation delay embryo development.
embryogenesis
hydrogen peroxide
sperm DNA damage
sperm chromatin fragmentation
Journal
Biology of reproduction
ISSN: 1529-7268
Titre abrégé: Biol Reprod
Pays: United States
ID NLM: 0207224
Informations de publication
Date de publication:
15 Nov 2023
15 Nov 2023
Historique:
received:
17
04
2023
revised:
21
07
2023
accepted:
29
08
2023
medline:
17
11
2023
pubmed:
4
9
2023
entrez:
2
9
2023
Statut:
ppublish
Résumé
We previously demonstrated that MnCl2 induces double-stranded DNA breaks in sperm in a process that we term as sperm chromatin fragmentation. Here, we tested if the levels of double-stranded DNA breaks were corelated to the concentration of MnCl2, and we compared this to another agent that causes single-stranded DNA breaks, H2O2. We found that both methods have the advantage of inducing DNA breaks in a concentration-dependent manner. Mouse sperm were treated with varying concentrations of either H2O2 or MnCl2, and the DNA damage was assessed by pulse-field gel electrophoresis, and the alkaline and neutral comet assays. Oocytes were injected with either treated sperm and the resulting embryos analyzed with an embryoscope to detect subtle changes in embryonic development. We confirmed that H2O2 treatment induced primarily single-stranded DNA breaks and MnCl2 induced primarily double-stranded DNA breaks, indicating different mechanisms of damage. These sperm were injected into oocytes, and the development of the resulting embryos followed with an embryoscope equipped with time lapse recording. We found that aberrations in early embryonic development by day 2 with even the lowest levels of DNA damage and that the levels of embryonic aberrations correlated to the concentration of either H2O2 or MnCl2. Low levels of H2O2 caused significantly more aberrations in embryonic development than low levels of MnCl2 even though the levels of DNA damage as measured by comet assays were similar. These data demonstrate that even low levels of sperm DNA damage cause delays and arrests in embryonic development.
Identifiants
pubmed: 37658763
pii: 7258932
doi: 10.1093/biolre/ioad106
pmc: PMC10651064
doi:
Substances chimiques
Chromatin
0
Hydrogen Peroxide
BBX060AN9V
manganese chloride
QQE170PANO
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
635-643Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM131944
Pays : United States
Organisme : NIH HHS
ID : GM123048
Pays : United States
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of Society for the Study of Reproduction.
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