Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique.
Model Organisms
Molecular Biology
Molecular/Chemical Probes
Neuroscience
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
02 Sep 2023
02 Sep 2023
Historique:
received:
30
05
2023
revised:
24
07
2023
accepted:
10
08
2023
medline:
3
9
2023
pubmed:
3
9
2023
entrez:
3
9
2023
Statut:
aheadofprint
Résumé
Analysis of synaptic strength and plasticity provides functional insights of complicated neural circuits. Here, we describe steps for cell- and projection-specific optogenetic manipulation of divergent basal ganglia circuits using anterograde and retrograde viral vectors. We quantitatively analyze synaptic function of these circuits utilizing a patch-clamp technique. This protocol is applicable to probe potential circuit targets for treatment of brain diseases. For complete details on the use and execution of this protocol, please refer to Ji et al.
Identifiants
pubmed: 37660296
pii: S2666-1667(23)00518-X
doi: 10.1016/j.xpro.2023.102551
pmc: PMC10491855
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102551Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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