Expression data of FOS and JUN genes and FTIR spectra provide diagnosis of thyroid carcinoma.

Diagnosis FOS FTIR Gene expression JUN Thyroid cancer

Journal

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN: 1873-3557
Titre abrégé: Spectrochim Acta A Mol Biomol Spectrosc
Pays: England
ID NLM: 9602533

Informations de publication

Date de publication:
05 Jan 2024
Historique:
received: 08 05 2023
revised: 11 08 2023
accepted: 26 08 2023
pubmed: 4 9 2023
medline: 4 9 2023
entrez: 3 9 2023
Statut: ppublish

Résumé

We explore the feasibility of using FOS and JUN gene expression and ATR-FTIR for diagnosis of thyroid cancer. For the study, 38 samples (6 non-neoplastic (NN), 10 papillary thyroid carcinoma (PTC), 7 follicular thyroid carcinoma (FTC), and 15 benign tumors (BT) were subjected to RNA extraction followed by quantitative real time PCR (qRT-PCR) and 30 samples (5 NN, 9 PTC, 5 FTC, and 11 BT) were used for Attenuated Total Reflectance - Fourier Transform Infrared (ATR-FTIR) followed by multivariate analysis. Of the above, 20 samples were used for both gene expression and ATR-FTIR studies. We found FOS and JUN expression in malignant tumor samples to be significantly lower than NN and benign. ATR-FIR after multivariate analysis could identify the difficult to diagnose FTC with 93 % efficiency. Overall, results suggest the diagnostic potential of molecular biology techniques combined with ATR-FTIR spectroscopy in differentiated thyroid carcinomas (PTC and FTC) and BT.

Identifiants

DOI: 10.1016/j.saa.2023.123305 PMID: 37660502
pubmed: 37660502
pii: S1386-1425(23)00990-3
doi: 10.1016/j.saa.2023.123305
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

123305

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Renata de Azevedo Canevari reports financial support was provided by State of Sao Paulo Research Foundation.

Auteurs

João Paulo da Silva Queiroz (JP)

Laboratório de Biologia Molecular do Câncer, Universidade do Vale do Paraíba, UNIVAP, Instituto de Pesquisa e Desenvolvimento, Avenida Shishima Hifumi 2911, Urbanova, São José dos Campos, 12244-000 São Paulo, SP, Brazil.

Breno Pupin (B)

Laboratório de Biologia Molecular do Câncer, Universidade do Vale do Paraíba, UNIVAP, Instituto de Pesquisa e Desenvolvimento, Avenida Shishima Hifumi 2911, Urbanova, São José dos Campos, 12244-000 São Paulo, SP, Brazil.

Tanmoy Tapobrata Bhattacharjee (TT)

Sir John Walsh Research Institute, University of Otago, 310 Great King St, North Dunedin, Dunedin 9016, New Zealand.

Miyuki Uno (M)

Centro de Investigação Translacional em Oncologia, Departamento de Radiologia e Oncologia, Instituto do Cancer do Estado de São Paulo (ICESP), Faculdade de Medicina da Universidade de São Paulo (FMUSP), Avenida Dr. Arnaldo 251, Cerqueira César, São Paulo 01246-000, São Paulo, Brazil.

Roger Chammas (R)

Centro de Investigação Translacional em Oncologia, Departamento de Radiologia e Oncologia, Instituto do Cancer do Estado de São Paulo (ICESP), Faculdade de Medicina da Universidade de São Paulo (FMUSP), Avenida Dr. Arnaldo 251, Cerqueira César, São Paulo 01246-000, São Paulo, Brazil.

Marco Aurélio Vamondes Kulcsar (MA)

Serviço de Cirurgia de cabeça e Pescoço, Instituto do Câncer do Estado de São Paulo - ICESP, Av. Doutor Arnaldo, 251, Cerqueira César, CEP 01246-000 São Paulo, SP, Brazil.

Renata de Azevedo Canevari (R)

Laboratório de Biologia Molecular do Câncer, Universidade do Vale do Paraíba, UNIVAP, Instituto de Pesquisa e Desenvolvimento, Avenida Shishima Hifumi 2911, Urbanova, São José dos Campos, 12244-000 São Paulo, SP, Brazil. Electronic address: rcanevari@univap.br.

Classifications MeSH