Systems analysis reveals differential expression of endocervical genes in African women randomized to DMPA-IM, LNG implant or cu-IUD.

Contraception Female genital tract Mucosa Randomized clinical trial Reproductive health Transcriptome

Journal

Clinical immunology (Orlando, Fla.)
ISSN: 1521-7035
Titre abrégé: Clin Immunol
Pays: United States
ID NLM: 100883537

Informations de publication

Date de publication:
Oct 2023
Historique:
received: 18 07 2023
accepted: 22 08 2023
medline: 16 11 2023
pubmed: 4 9 2023
entrez: 3 9 2023
Statut: ppublish

Résumé

Although effective contraceptives are crucial for preventing unintended pregnancies, evidence suggests that their use may perturb the female genital tract (FGT). A comparative analysis of the effects of the most common contraceptives on the FGT have not been evaluated in a randomized clinical trial setting. Here, we evaluated the effect of three long-acting contraceptive methods: depot medroxyprogesterone acetate(DMPA-IM), levonorgestrel(LNG) implant, and a copper intrauterine device (Cu-IUD), on the endocervical host transcriptome in 188 women from the Evidence for Contraceptive Options and HIV Outcomes Trial (ECHO) trial. Cu-IUD usage showed the most extensive transcriptomic changes, and was associated with inflammatory and anti-viral host responses. DMPA-IM usage was enriched for pathways associated with T cell responses. LNG implant had the mildest effect on endocervical gene expression, and was associated with growth factor signaling. These data provide a mechanistic basis for the diverse influence that varying contraceptives have on the FGT.

Identifiants

pubmed: 37660744
pii: S1521-6616(23)00513-2
doi: 10.1016/j.clim.2023.109750
pmc: PMC10570927
pii:
doi:

Substances chimiques

Copper 789U1901C5
Levonorgestrel 5W7SIA7YZW
Contraceptive Agents 0

Types de publication

Randomized Controlled Trial Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109750

Subventions

Organisme : NIGMS NIH HHS
ID : T32 GM142617
Pays : United States

Informations de copyright

Copyright © 2023. Published by Elsevier Inc.

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Auteurs

Prachi Mehrotra Gupta (PM)

Emory National Primate Research Center (ENPRC) Genomics Core Laboratory, Division of Microbiology & Immunology, Emory University, Atlanta, GA, USA.

Christina Balle (C)

Department of Pathology, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa.

Gregory K Tharp (GK)

Emory National Primate Research Center (ENPRC) Genomics Core Laboratory, Division of Microbiology & Immunology, Emory University, Atlanta, GA, USA.

Sydney A Nelson (SA)

Emory National Primate Research Center (ENPRC) Genomics Core Laboratory, Division of Microbiology & Immunology, Emory University, Atlanta, GA, USA.

Melanie A Gasper (MA)

Seattle Children's Research Institute, Seattle, WA, USA.

Bryan Brown (B)

Seattle Children's Research Institute, Seattle, WA, USA; Department of Pediatrics, University of Washington, Seattle, WA, USA.

Arghavan Alisoltani (A)

Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

Maricianah Onono (M)

Kenya Medical Research Institute, Kisumu, Kenya.

Thesla Palanee-Phillips (T)

Wits RHI, University of the Witwatersrand, Faculty of Health Sciences, School of Public Health, Johannesburg, South Africa; Department of Epidemiology, School of Public Health, University of Washington, Seattle, WA, USA.

Gonsagrie Nair (G)

Desmond Tutu HIV Centre, Cape Town, South Africa.

Hosseana Ayele (H)

Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH, USA.

Laura Noel-Romas (L)

Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH, USA.

Jo-Ann S Passmore (JS)

Department of Pathology, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa; National Health Laboratory Service, Cape Town, South Africa; CAPRISA DSI-NRF Centre of Excellence in HIV Prevention, University of Cape Town, South Africa.

Adam D Burgener (AD)

Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH, USA.

Renee Heffron (R)

Department of Epidemiology, School of Public Health, University of Washington, Seattle, WA, USA; Department of Global Health, University of Washington, Seattle, WA, USA.

Heather B Jaspan (HB)

Department of Pathology, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa; Seattle Children's Research Institute, Seattle, WA, USA; Department of Pediatrics, University of Washington, Seattle, WA, USA; Department of Global Health, University of Washington, Seattle, WA, USA.

Steven E Bosinger (SE)

Emory University School of Medicine, Department of Pathology & Laboratory Medicine, GA, USA; Emory Vaccine Center, Emory University, GA, USA. Electronic address: steven.bosinger@emory.edu.

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