Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem.

Human milk oligosaccharide In vitro synthetic enzymatic biosystem Lacto-N-biose I Thermostable enzyme

Journal

Synthetic and systems biotechnology
ISSN: 2405-805X
Titre abrégé: Synth Syst Biotechnol
Pays: China
ID NLM: 101694371

Informations de publication

Date de publication:
Sep 2023
Historique:
received: 14 04 2023
revised: 07 08 2023
accepted: 16 08 2023
medline: 4 9 2023
pubmed: 4 9 2023
entrez: 4 9 2023
Statut: epublish

Résumé

Human milk oligosaccharides (HMOs) are very distinctive components in human milk and are beneficial for infant health. Lacto-N-biose I (LNB) is the core structural unit of HMOs, which could be used for the synthesis of other HMOs. In this study, an ATP-free in vitro synthetic enzymatic biosystem contained four thermostable enzymes (alpha-glucan phosphorylase from

Identifiants

pubmed: 37663063
doi: 10.1016/j.synbio.2023.08.004
pii: S2405-805X(23)00061-3
pmc: PMC10468323
doi:

Types de publication

Journal Article

Langues

eng

Pagination

555-562

Informations de copyright

© 2023 The Authors.

Déclaration de conflit d'intérêts

The authors declare that they have no conflicts of interest in this study.

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Auteurs

Lijie Chen (L)

College of Bioengineering, Tianjin University of Science and Technology, Tianjin, 300457, China.
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

Yanmei Qin (Y)

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.

Long Ma (L)

College of Bioengineering, Tianjin University of Science and Technology, Tianjin, 300457, China.

Dongdong Meng (D)

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

Chun You (C)

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
National Center of Technology Innovation for Synthetic Biology, Tianjin, 300308, China.

Classifications MeSH