Combating pan-coronavirus infection by indomethacin through simultaneously inhibiting viral replication and inflammatory response.

Drugs Immune response Virology

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
15 Sep 2023
Historique:
received: 25 06 2023
revised: 05 07 2023
accepted: 11 08 2023
medline: 4 9 2023
pubmed: 4 9 2023
entrez: 4 9 2023
Statut: epublish

Résumé

Severe infections with coronaviruses are often accompanied with hyperinflammation, requiring therapeutic strategies to simultaneously tackle the virus and inflammation. By screening a safe-in-human broad-spectrum antiviral agents library, we identified that indomethacin can inhibit pan-coronavirus infection in human cell and airway organoids models. Combining indomethacin with oral antiviral drugs authorized for treating COVID-19 results in synergistic anti-coronavirus activity. Coincidentally, screening a library of FDA-approved drugs identified indomethacin as the most potent potentiator of interferon response through increasing STAT1 phosphorylation. Combining indomethacin with interferon-alpha exerted synergistic antiviral effects against multiple coronaviruses. The anti-coronavirus activity of indomethacin is associated with activating interferon response. In a co-culture system of lung epithelial cells with macrophages, indomethacin inhibited both viral replication and inflammatory response. Collectively, indomethacin is a pan-coronavirus inhibitor that can simultaneously inhibit virus-triggered inflammatory response. The therapeutic potential of indomethacin can be further augmented by combining it with oral antiviral drugs or interferon-alpha.

Identifiants

pubmed: 37664584
doi: 10.1016/j.isci.2023.107631
pii: S2589-0042(23)01708-X
pmc: PMC10474465
doi:

Types de publication

Journal Article

Langues

eng

Pagination

107631

Informations de copyright

© 2023 The Author(s).

Déclaration de conflit d'intérêts

The authors disclose no conflicts.

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Auteurs

Yining Wang (Y)

Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, the Netherlands.

Pengfei Li (P)

Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, the Netherlands.

Lei Xu (L)

Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, the Netherlands.
State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.

Annemarie C de Vries (AC)

Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, the Netherlands.

Robbert J Rottier (RJ)

Department of Pediatric Surgery, Erasmus MC-Sophia Children's Hospital, Rotterdam, the Netherlands.
Department of Cell Biology, Erasmus MC-University Medical Center, Rotterdam, the Netherlands.

Wenshi Wang (W)

Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, the Netherlands.
Jiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, China.

Marie-Rose B S Crombag (MBS)

Department of Hospital Pharmacy, Erasmus MC-University Medical Center, Rotterdam, the Netherlands.

Maikel P Peppelenbosch (MP)

Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, the Netherlands.

Denis E Kainov (DE)

Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, 7028 Trondheim, Norway.
Institute of Technology, University of Tartu, 50090 Tartu, Estonia.

Qiuwei Pan (Q)

Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, the Netherlands.

Classifications MeSH