High-CBD cannabis extracts inhibit the expression of proinflammatory factors via miRNA-mediated silencing in human small intestinal epithelial cells.
Cannabis extract
IL-6
IL-8
miR-302c-3p
miR-760
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
01
09
2022
revised:
26
07
2023
accepted:
28
07
2023
medline:
4
9
2023
pubmed:
4
9
2023
entrez:
4
9
2023
Statut:
epublish
Résumé
The incidence of chronic inflammatory disorders and autoimmune diseases is rapidly growing. To date, the COVID-19 pandemic caused by SARS-CoV-2 has killed over 6,209,000 people globally, while no drug has been proven effective for the disease. Screening natural anti-inflammatory compounds for clinical application has drawn much attention. In this study, we showed that high-CBD cannabis extracts #1, #5, #7, #169, and #317 suppressed the levels of expression of proinflammatory cyclooxygenase 2 (COX2) and increased the expression of the anti-inflammatory suppressor of cytokine signaling 3 (SOCS3) in human small intestinal epithelial cells (HSIEC) in TNFα/IFNγ-triggered inflammation. We revealed that these extracts, with the exception of extract #169, also profoundly attenuated induction of proinflammatory cytokines interleukin-6 (IL-6) and/or IL-8 proteins through miR-760- and miR-302c-3p-mediated silencing. The prevalent components in extracts #1 and #7 influenced the levels of IL-8 both individually as well as in combination with each other. However, the high-dose cannabis extracts displayed an inhibitory effect in the growth of HSIEC cells. These results show that our high-CBD cannabis extracts decrease the levels of proinflammatory molecules COX2, IL-6, and IL-8 via transcriptional suppression or miRNA-mediated silencing, highlighting their potential against COVID-19-associated cytokine storm syndrome.
Identifiants
pubmed: 37664748
doi: 10.1016/j.heliyon.2023.e18817
pii: S2405-8440(23)06025-5
pmc: PMC10468390
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e18817Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare the following conflict of interests: Pathway Rx and Swysh are biotechnology start-ups involved in medical cannabis, health and disease research and development. Notwithstanding, this had no. bearing on the study design, data analysis and interpretation. O.K., A.F and I.K are co-founders of start-ups, and B.W. and D.L. are team members. All listed authors have filed for the following relevant patents: PCT/IL2021/050265 and USP no. 62/994873. Authors have applied for other patents in medical cannabis field, albeit these are unrelated to the current study.
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