Immunohistochemical investigation of parainfluenza 3 virus in sheep pneumonia.
Histopathology
Immunohistochemistry
Parainfluenza 3
Pneumonia
Sheep
Journal
Veterinary research forum : an international quarterly journal
ISSN: 2008-8140
Titre abrégé: Vet Res Forum
Pays: Iran
ID NLM: 101625812
Informations de publication
Date de publication:
2023
2023
Historique:
received:
13
04
2022
accepted:
01
08
2022
medline:
5
9
2023
pubmed:
5
9
2023
entrez:
5
9
2023
Statut:
ppublish
Résumé
The aim of this study was to determine the prevalence of parainfluenza 3 (PI3) virus antigen through histopathological and immunohistochemical (IHC) methods in sheep lung samples collected from Erzurum province, Türkiye. Between August and November 2017, 1462 sheep were dissected in the slaughterhouse and their lungs were examined macroscopically. In total, 100 of the lung samples with pneumonia were selected. Routine histopathological and IHC analyses of the collected lung tissues with pneumonia were performed. Pneumonia observed through macroscopical and histopathological examinations of the lung samples was classified as purulent-catarrhal bronchopneumonia (14.00%), fibrinous bronchopneumonia (23.00%), interstitial pneumonia (69.00%), granulomatous pneumonia (7.00%), verminous pneumonia (19.00%) and pulmonary adenomatosis (6.00%). Two or three types of pneumonia were observed in many of the same cases. The PI3 virus antigen positivity rate in the IHC analysis of sheep lung samples was 19.00%. In the IHC tracing, positivities were found mostly in the alveolar macrophages and cytoplasm of bronchial, bronchiolar and alveolar epithelial cells. As a result, the prevalence of PI3 virus in sheep in Erzurum province, Türkiye, was determined to be 19.00% using KLN BVB IHC method.
Identifiants
pubmed: 37667793
doi: 10.30466/vrf.2022.551919.3447
pmc: PMC10475171
doi:
Types de publication
Journal Article
Langues
eng
Pagination
461-464Informations de copyright
© 2023 Urmia University. All rights reserved.
Déclaration de conflit d'intérêts
There is no conflict of interest between the authors.
Références
Transbound Emerg Dis. 2019 May;66(3):1411-1416
pubmed: 30770651
J Vet Med. 2019 Jul 18;2019:5169040
pubmed: 31396540
J Immunoassay Immunochem. 2017;38(5):555-568
pubmed: 28679075
Virus Res. 2016 Sep 2;223:80-7
pubmed: 27374058
J Virol Methods. 2015 Sep 15;222:47-54
pubmed: 26031225
Antiviral Res. 2020 Dec;184:104903
pubmed: 32800881
Arch Virol. 2019 Jul;164(7):1967-1980
pubmed: 31089958
Viruses. 2019 May 29;11(6):
pubmed: 31146368
Virusdisease. 2017 Mar;28(1):102-110
pubmed: 28466061
Pol J Vet Sci. 2021 Jun;24(2):235-241
pubmed: 34250778