Effects of prenatal Doppler ultrasound on the retina of the chick embryo in ovo.
chicken embryo
prenatal ultrasound
retinal development
Journal
Anatomia, histologia, embryologia
ISSN: 1439-0264
Titre abrégé: Anat Histol Embryol
Pays: Germany
ID NLM: 7704218
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
03
05
2023
accepted:
21
08
2023
medline:
14
11
2023
pubmed:
5
9
2023
entrez:
5
9
2023
Statut:
ppublish
Résumé
Currently, the effect of prenatal ultrasound on foetal development is intensively discussed and the guidelines for prenatal diagnostics have been changed. However, data supporting these concerns are scarce. Therefore, we used an established in ovo model of the chicken embryo to investigate cell proliferation and apoptosis within the retina. A total of 21 chicken eggs were fenestrated on Day 5 and allocated to either the control (n = 8) or exposition group (n = 13). The exposition group was treated with pulsed-wave Doppler ultrasound (PWD) for 10 min while controls remained without treatment. After subsequent incubation (6-48 h), the eggs were sacrificed, and chicken embryos were examined morphologically (HE-staining) and immunohistochemically. Counting of apoptotic and proliferating cells per retina was performed using antibodies specific for phospho-histone-H3 and active caspase-3 in combination with a biotin-labelled secondary antibody and peroxidase conjugated avidin-biotin complex for chromogenic detection. Due to a rather low number of specimens at each time point after ultrasound exposition, we neglected the effects of incubation time and focused on treatment effects. This approach revealed that the median number of proliferating cells is reduced after 10 min of exposure to PWD (569 vs. 766), while the number of apoptotic cells is fairly comparable between groups (5 vs. 6). Our data contribute to a better understanding of prenatal US on foetal development by suggesting that PWD could have an impact on the number of proliferating cells in the developing chicken retina and therefore justify further investigations.
Substances chimiques
Biotin
6SO6U10H04
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1003-1009Informations de copyright
© 2023 The Authors. Anatomia, Histologia, Embryologia published by Wiley-VCH GmbH.
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