Mimicking superinfection exclusion disrupts alphavirus infection and transmission in the yellow fever mosquito

alphaviruses arbovirus transmission nsP2 protease superinfection exclusion

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
12 09 2023
Historique:
medline: 7 9 2023
pubmed: 5 9 2023
entrez: 5 9 2023
Statut: ppublish

Résumé

Multiple viruses, including pathogenic viruses, bacteriophages, and even plant viruses, cause a phenomenon termed superinfection exclusion whereby a currently infected cell is resistant to secondary infection by the same or a closely related virus. In alphaviruses, this process is thought to be mediated, at least in part, by the viral protease (nsP2) which is responsible for processing the nonstructural polyproteins (P123 and P1234) into individual proteins (nsP1-nsP4), forming the viral replication complex. Taking a synthetic biology approach, we mimicked this naturally occurring phenomenon by generating a superinfection exclusion-like state in

Identifiants

pubmed: 37669371
doi: 10.1073/pnas.2303080120
pmc: PMC10500260
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2303080120

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007033
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007038
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007039
Pays : United Kingdom

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Auteurs

Christine M Reitmayer (CM)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Emily Levitt (E)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Sanjay Basu (S)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Barry Atkinson (B)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Rennos Fragkoudis (R)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Andres Merits (A)

Applied Virology, Institute of Technology, University of Tartu, Tartu 50411, Estonia.

Sarah Lumley (S)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Will Larner (W)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Adriana V Diaz (AV)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Sara Rooney (S)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Callum J E Thomas (CJE)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Katharina von Wyschetzki (K)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

Kai Rausalu (K)

Applied Virology, Institute of Technology, University of Tartu, Tartu 50411, Estonia.

Luke Alphey (L)

Arthropod Genetics, The Pirbright Institute, Pirbright, Woking GU24 0NF, United Kingdom.

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Classifications MeSH