Impact of active lifestyle on the primary school children saliva microbiota composition.
children
exercise
lifestyle
microbiota
saliva
Journal
Frontiers in nutrition
ISSN: 2296-861X
Titre abrégé: Front Nutr
Pays: Switzerland
ID NLM: 101642264
Informations de publication
Date de publication:
2023
2023
Historique:
received:
26
05
2023
accepted:
28
07
2023
medline:
6
9
2023
pubmed:
6
9
2023
entrez:
6
9
2023
Statut:
epublish
Résumé
The aim of the study was to evaluate the effects of Active or Sedentary lifestyle on saliva microbiota composition in Italian schoolchildren. Male (114) and female children (8-10 years) belonging to five primary schools in the neighborhoods of Turin were classified as active (A) or sedentary (S) based on PAQ-C-It questionnaire. PCR amplification of salivary DNA targeted the hypervariable V3-V4 regions of the 16S rRNA bacterial genes. DADA2 workflow was used to infer the Amplicon Sequence Variants and the taxonomic assignments; the beta-diversity was obtained by PCoA with the UniFrac method; LEfSe algorithm, threshold at 5%, and Log LDA cutoff at ±0.5 were used to identify differently abundant species in A compared to S saliva sample. Daily food intake was assessed by 3-Days food record. The metabolic potential of microbial communities was assessed by PICRUSt. No significant differences were found in individual's gender distribution ( Our results showed that active children had an enrichment of species and genera mainly associated with a healthier profile. By contrast, the genera and the species enriched in the sedentary group could be linked to human diseases.
Identifiants
pubmed: 37671197
doi: 10.3389/fnut.2023.1226891
pmc: PMC10476528
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1226891Informations de copyright
Copyright © 2023 Mancini, Cerulli, Vitucci, Lasorsa, Parente, Di Credico, Orrù, Brustio, Lupo, Rainoldi, Schena, Capasso and Buono.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer GC declared a past co-authorship with the author CC to the handling editor.
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