ST2-Conditioned Medium Fosters Dorsal Horn Cell Excitability and Synaptic Transmission in Cultured Mouse Spinal Cord.
Calcium imaging
Dorsal horn neurons
Firing activity
Mesenchymal stem/stromal cells
Spinal cord organotypic cultures
Journal
Stem cell reviews and reports
ISSN: 2629-3277
Titre abrégé: Stem Cell Rev Rep
Pays: United States
ID NLM: 101752767
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
accepted:
28
08
2023
medline:
27
11
2023
pubmed:
7
9
2023
entrez:
6
9
2023
Statut:
ppublish
Résumé
Conditioned medium obtained from bone marrow-derived stem cells has been proposed as a novel cell-free therapy in spinal cord injury and neuropathic pain, yet the direct effect on spinal neuron function has never been investigated. Here, we adopted spinal cord organotypic cultures (SCOCs) as an experimental model to probe the effect of ST2 murine mesenchymal stem cells-conditioned medium (ST2-CM) on dorsal horn (DH) neuron functional properties. Three days of SCOC exposure to ST2-CM increased neuronal activity measured by Fos expression, as well as spontaneous or induced firing. We showed that the increase in neuronal excitability was associated with changes in both intrinsic membrane properties and an enhanced excitatory drive. The increased excitability at the single-cell level was substantiated at the network level by detecting synchronous bursts of calcium waves across DH neurons. Altogether, SCOCs represent a viable tool to probe mesenchymal cells' effect on intact neuronal networks. Our findings indicate that ST2-CM enhances neuronal activity and synaptic wiring in the spinal dorsal horn. Our data also support the trophic role of mesenchymal cells CM in maintaining network activity in spinal circuits.
Identifiants
pubmed: 37674016
doi: 10.1007/s12015-023-10618-x
pii: 10.1007/s12015-023-10618-x
pmc: PMC10661801
doi:
Substances chimiques
Culture Media, Conditioned
0
Interleukin-1 Receptor-Like 1 Protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2918-2928Informations de copyright
© 2023. The Author(s).
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