Protein reservoirs of seeds are amyloid composites employed differentially for germination and seedling emergence.
Cicer arietinum
Hordeum vulgare
Triticum aestivum
Vigna radiata
amyloid composites in plants
amyloids in germination and seedling growth
functional amyloids
protein aggregates
seed storage protein bodies
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
revised:
15
07
2023
received:
17
05
2023
accepted:
07
08
2023
pubmed:
7
9
2023
medline:
7
9
2023
entrez:
7
9
2023
Statut:
ppublish
Résumé
Seed protein localization in seed storage protein bodies (SSPB) and their significance in germination are well recognized. SSPB are spherical and contain an assembly of water-soluble and salt-soluble proteins. Although the native structures of some SSPB proteins are explored, their structural arrangement to the functional correlation in SSPB remains unknown. SSPB are morphologically analogous to electron-dense amyloid-containing structures reported in other organisms. Here, we show that wheat, mungbean, barley, and chickpea SSPB exhibit a speckled pattern of amyloids interspersed in an amyloid-like matrix along with native structures, suggesting the composite nature of SSPB. This is confirmed by multispectral imaging methods, electron microscopy, infrared, and X-ray diffraction analysis, using in situ tissue sections, ex vivo protoplasts, and in vitro SSPB. Laser capture microdissection coupled with peptide fingerprinting has shown that globulin 1 and 3 in wheat, and 8S globulin and conglycinin in mungbean are the major amyloidogenic proteins. The amyloid composites undergo a sustained degradation during germination and seedling growth, facilitated by an intricate interplay of plant hormones and proteases. These results would lay down the foundation for understanding the amyloid composite structure during SSPB biogenesis and its evolution across the plant kingdom and have implications in both basic and applied plant biology.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
329-346Subventions
Organisme : Indian Institute of Technology Kanpur
ID : IITK/BSBE/20100293
Informations de copyright
© 2023 Society for Experimental Biology and John Wiley & Sons Ltd.
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