Stem cell challenges and opportunities.

Animals Mice Humans Hematopoietic Stem Cells Liver Pluripotent Stem Cells Biological Assay Cell Differentiation Disease Models, Animal
Hepatocyte-like cells chimeric mice colony-forming units in spleen drug-induced liver injury extramedullary erythropoiesis hematopoietic stem cells hepatic stem cell human pluripotent stem cells

Journal

Progress in molecular biology and translational science
ISSN: 1878-0814
Titre abrégé: Prog Mol Biol Transl Sci
Pays: Netherlands
ID NLM: 101498165

Informations de publication

Date de publication:
2023
Historique:
medline: 11 9 2023
pubmed: 8 9 2023
entrez: 7 9 2023
Statut: ppublish

Résumé

Hepatocyte-like cells (HLCs) generated from human pluripotent stem cells (PSCs) exhibit hepatocytic properties in vitro; however, their engraftment and functionality in vivo remain unsatisfactory. Despite optimization of differentiation protocols, HLCs did not engraft in a mouse model of liver injury. In contrast, organ-derived hepatocytes reproducibly formed colonies in the liver injury mouse model. As an extension of the phenomenon observed in hematopoietic stem cells giving rise to colonies within the spleen, commonly referred to as "colony-forming units in spleen (CFU-s)", we hypothesize that "colony-forming units in liver (CFU-L)" serves as a reliable indicator of stemness, engraftment, and functionality of hepatocytes. The uniform expression of the randomly inactivated gene in a single colony, as reported by Sugahara et al. 2022, suggests that the colonies generated by isolated hepatocytes likely originate from a single cell. We, therefore, propose that CFU-L can be used to quantify the number of "hepatocytes that engraft and proliferate in vivo" as a quantitative assay for stem cells that utilize colony-forming ability, similar to that observed in hematopoietic stem cells.

Identifiants

DOI: 10.1016/bs.pmbts.2023.03.004 PMID: 37678981
pubmed: 37678981
pii: S1877-1173(23)00063-7
doi: 10.1016/bs.pmbts.2023.03.004
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

379-395

Informations de copyright

Copyright © 2023. Published by Elsevier Inc.

Auteurs

Kenta Ite (K)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan; Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.

Masashi Toyoda (M)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan; Research team for Aging Science (Vascular Medicine), Tokyo Metropolitan Institute for Geriatrics and Gerontology, Tokyo, Japan.

Saeko Akiyama (S)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan; Department of Advanced Pediatric Medicine (National Center for Child Health and Development), Tohoku University School of Medicine, Miyagi, Japan.

Shin Enosawa (S)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan.

Saeko Yoshioka (S)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan.

Takaaki Yukitake (T)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan; Department of Applied Biological Science, Tokyo University of Science, Tokyo, Japan.

Mayu Yamazaki-Inoue (M)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan.

Kuniko Tatsumi (K)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan.

Hidenori Akutsu (H)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan.

Hiroshi Nishina (H)

Department of Developmental and Regenerative Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.

Toru Kimura (T)

Department of BioSciences, Kitasato University School of Science, Kanagawa, Japan.

Naoko Otani (N)

Department of Applied Biological Science, Tokyo University of Science, Tokyo, Japan.

Atsuko Nakazawa (A)

Department of Pathology, National Center for Child Health and Development Hospital, Tokyo, Japan.

Akinari Fukuda (A)

Department of Pathology, National Center for Child Health and Development Hospital, Tokyo, Japan.

Mureo Kasahara (M)

Department of Pathology, National Center for Child Health and Development Hospital, Tokyo, Japan.

Akihiro Umezawa (A)

Center for Regenerative Medicine, National Center for Child Health and Development Research Institute, Tokyo, Japan; Department of Advanced Pediatric Medicine (National Center for Child Health and Development), Tohoku University School of Medicine, Miyagi, Japan. Electronic address: umezawa@1985.jukuin.keio.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Stem cell challenges and opportunities.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Stem cell challenges and opportunities.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Stem cell challenges and opportunities.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système hématopoïétique › Cellules de la moelle osseuse › Cellules souches hématopoïétiques › Stem cell challenges and opportunities.
questionsmedicales.fr › Système digestif › Foie › Stem cell challenges and opportunities.
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Stem cell challenges and opportunities.
questionsmedicales.fr › Techniques d'investigation › Dosage biologique › Stem cell challenges and opportunities.
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Stem cell challenges and opportunities.
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Stem cell challenges and opportunities.