Mechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach.
CHO
S-sulfocysteine
biological activity
omics
redox control
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2023
2023
Historique:
received:
28
05
2023
accepted:
31
07
2023
medline:
8
9
2023
pubmed:
8
9
2023
entrez:
8
9
2023
Statut:
epublish
Résumé
S-Sulfocysteine (SSC), a bioavailable L-cysteine derivative (Cys), is known to be taken up and metabolized in Chinese hamster ovary (CHO) cells used to produce novel therapeutic biological entities. To gain a deeper mechanistic insight into the SSC biological activity and metabolization, a multi-omics study was performed on industrially relevant CHO-K1 GS cells throughout a fed-batch process, including metabolomic and proteomic profiling combined with multivariate data and pathway analyses. Multi-layered data and enzymatical assays revealed an intracellular SSC/glutathione mixed disulfide formation and glutaredoxin-mediated reduction, releasing Cys and sulfur species. Increased Cys availability was directed towards glutathione and taurine synthesis, while other Cys catabolic pathways were likewise affected, indicating that cells strive to maintain Cys homeostasis and cellular functions.
Identifiants
pubmed: 37680342
doi: 10.3389/fbioe.2023.1230422
pii: 1230422
pmc: PMC10482334
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1230422Informations de copyright
Copyright © 2023 Nguyen, Le Mignon, Schnellbächer, Wehsling, Braun, Baumgaertner, Grabner and Zimmer.
Déclaration de conflit d'intérêts
Authors MN, ML, AS, MW, JuB, MG, and AZ were employed by the company Merck Life Science KGaA. Author JeB was employed by the company Merck KGaA.
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