Drosophila X virus-like particles as delivery carriers for improved oral insecticidal efficacy of scorpion Androctonus australis peptide against the invasive fruit fly, Drosophila suzukii.
AaIT
Drosophila suzukii
nanocarrier
neurotoxic peptides
pest control
virus-like particle (VLP)
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
08 Sep 2023
08 Sep 2023
Historique:
revised:
01
08
2023
received:
11
04
2023
accepted:
04
08
2023
pubmed:
8
9
2023
medline:
8
9
2023
entrez:
8
9
2023
Statut:
aheadofprint
Résumé
Insect-specific neurotoxic peptides derived from the venoms of scorpions and spiders can cause acute paralysis and death when injected into insects, offering a promising insecticidal component for insect pest control. However, effective delivery systems are required to help neurotoxic peptides pass through the gut barrier into the hemolymph, where they can act. Here, we investigated the potential of a novel nanocarrier, Drosophila X virus-like particle (DXV-VLP), for delivering a neurotoxin from the scorpion Androctonus australis Hector (AaIT) against the invasive pest fruit fly, Drosophila suzukii. Our results show that the fusion proteins of DXV polyproteins with AaIT peptide at their C-termini could be sufficiently produced in Lepidoptera Hi5 cells in a soluble form using the recombinant baculovirus expression system, and could self-assemble into VLPs with similar particle morphology and size to authentic DXV virions. In addition, the AaIT peptides displayed on DXV-VLPs retained their toxicity, as demonstrated in injection bioassays that resulted in severe mortality (72%) in adults after 72 h. When fed to adults, mild mortality was observed in the group treated with DXV-AaIT (38%), while no mortality occurred in the group treated with AaIT peptide, thus indicating the significant role of DXV-VLPs in delivering AaIT peptides. Overall, this proof-of-concept study demonstrates for the first time that VLPs can be exploited to enhance oral delivery of insect-specific neurotoxic peptides in the context of pest control. Moreover, it provides insights for further improvements and potentially the development of neurotoxin-based bioinsecticides and/or transgenic crops for insect pest control.
Identifiants
pubmed: 37681406
doi: 10.1111/1744-7917.13271
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : China Scholarship Council
ID : Doctoral scholarship
Organisme : Bijzonder Onderzoeksfonds UGent
Organisme : Hellenic Foundation for Research and Innovation
ID : VLP-RNAi"
Organisme : Hellenic Foundation for Research and Innovation
ID : Project Number: 785
Organisme : Fonds Wetenschappelijk Onderzoek
ID : 12V5722N
Informations de copyright
© 2023 Institute of Zoology, Chinese Academy of Sciences.
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