Genetic diversity and wing geometric morphometrics among four populations of Aedes aegypti (Diptera: Culicidae) from Benin.
Aedes aegypti
Africa
Benin
Genetics
Morphometry
Population structure
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
09 Sep 2023
09 Sep 2023
Historique:
received:
06
07
2023
accepted:
24
08
2023
medline:
11
9
2023
pubmed:
9
9
2023
entrez:
8
9
2023
Statut:
epublish
Résumé
The impact of the arbovirus vector Aedes aegypti is of major concern for global public health as the viruses that it transmits affect millions of people each year worldwide. Originating in Africa, Ae. aegypti has now spread throughout much of the world. While the genetic makeup of Ae. aegypti in the New World has been extensively studied, there is limited knowledge on its genetic diversity in Africa, particularly at a microgeographical level. We investigated mitochondrial cytochrome oxidase I of four Ae. aegypti populations from Benin and employed wing morphometric analyses as a cost-effective and reliable tool to explore population structure. Our sampling encompassed various areas of Benin, from the southern to the northern borders of the country, and included urban, semi-urban, and sylvatic sites. We observed a notable level of genetic diversity (haplotype diversity of 0.8333) and nucleotide diversity (0.00421986), and identified seven distinct haplotypes. Sylvatic and semi-urban sites exhibited a greater number of haplotypes compared to urban sites. Utilizing 18 wing landmarks, we calculated the centroid size, which revealed significant variation among the three landscape types. However, principal component analysis, employed to assess wing shape variation, did not demonstrate significant differences between populations based on landscape type. Our findings indicate substantial genetic and morphological diversity among Ae. aegypti populations in Benin, and provide insight into important biological characteristics of these populations with respect to their potential to transmit viruses. To the best of our knowledge, this is the first study undertaken in Africa to integrate genetics with morphology to analyse the population structure of the major arbovirus vector Ae. aegypti.
Sections du résumé
BACKGROUND
BACKGROUND
The impact of the arbovirus vector Aedes aegypti is of major concern for global public health as the viruses that it transmits affect millions of people each year worldwide. Originating in Africa, Ae. aegypti has now spread throughout much of the world. While the genetic makeup of Ae. aegypti in the New World has been extensively studied, there is limited knowledge on its genetic diversity in Africa, particularly at a microgeographical level.
METHODS
METHODS
We investigated mitochondrial cytochrome oxidase I of four Ae. aegypti populations from Benin and employed wing morphometric analyses as a cost-effective and reliable tool to explore population structure. Our sampling encompassed various areas of Benin, from the southern to the northern borders of the country, and included urban, semi-urban, and sylvatic sites.
RESULTS
RESULTS
We observed a notable level of genetic diversity (haplotype diversity of 0.8333) and nucleotide diversity (0.00421986), and identified seven distinct haplotypes. Sylvatic and semi-urban sites exhibited a greater number of haplotypes compared to urban sites. Utilizing 18 wing landmarks, we calculated the centroid size, which revealed significant variation among the three landscape types. However, principal component analysis, employed to assess wing shape variation, did not demonstrate significant differences between populations based on landscape type.
CONCLUSIONS
CONCLUSIONS
Our findings indicate substantial genetic and morphological diversity among Ae. aegypti populations in Benin, and provide insight into important biological characteristics of these populations with respect to their potential to transmit viruses. To the best of our knowledge, this is the first study undertaken in Africa to integrate genetics with morphology to analyse the population structure of the major arbovirus vector Ae. aegypti.
Identifiants
pubmed: 37684701
doi: 10.1186/s13071-023-05943-6
pii: 10.1186/s13071-023-05943-6
pmc: PMC10492319
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
320Subventions
Organisme : German Research Foundation
ID : JO 1276/5-1
Organisme : German Research Foundation
ID : JO 1276/5-1
Organisme : German Research Foundation
ID : JO 1276/5-1
Organisme : Federal Ministry of Education and Research of Germany
ID : 01Kl2022
Organisme : Federal Ministry of Education and Research of Germany
ID : 01Kl2022
Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
Références
Mil Med Res. 2022 Nov 14;9(1):64
pubmed: 36372882
Parasit Vectors. 2016 Apr 23;9:228
pubmed: 27108406
Bioinformatics. 2009 Jun 1;25(11):1451-2
pubmed: 19346325
Infect Genet Evol. 2010 May;10(4):580-5
pubmed: 20123039
Mol Ecol. 2019 Sep;28(18):4138-4151
pubmed: 31482608
Nature. 2014 Nov 13;515(7526):222-7
pubmed: 25391959
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Mem Inst Oswaldo Cruz. 2013;108 Suppl 1:11-7
pubmed: 24473798
Pathogens. 2022 May 26;11(6):
pubmed: 35745474
Mol Ecol. 2016 Nov;25(21):5377-5395
pubmed: 27671732
Dev Genes Evol. 2000 Dec;210(12):617-22
pubmed: 11151298
Parasit Vectors. 2018 Oct 26;11(1):561
pubmed: 30367678
Trop Med Health. 2020 Apr 10;48:20
pubmed: 32308531
Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9
pubmed: 7881515
Ecol Evol. 2018 Jul 13;8(16):7835-7848
pubmed: 30250667
Int J Environ Res Public Health. 2020 Feb 21;17(4):
pubmed: 32098137
Front Genet. 2023 Mar 09;14:1107893
pubmed: 36968606
Infect Genet Evol. 2013 Jan;13:242-51
pubmed: 22985681
Acta Trop. 2019 Mar;191:162-171
pubmed: 30529448
J Arthropod Borne Dis. 2018 Dec 25;12(4):351-360
pubmed: 30918904
Infect Genet Evol. 2008 Dec;8(6):875-90
pubmed: 18832048
Nat Rev Genet. 2016 Jul;17(7):422-33
pubmed: 27265362
Bioscience. 2018 Nov 1;68(11):854-860
pubmed: 30464351
Infect Genet Evol. 2017 Oct;54:205-215
pubmed: 28673547
J Med Entomol. 2007 Sep;44(5):788-95
pubmed: 17915509
Curr Biol. 2020 Sep 21;30(18):3570-3579.e6
pubmed: 32707056
Parasit Vectors. 2015 Feb 26;8:128
pubmed: 25885902
Ann Trop Med Parasitol. 1957 Dec;51(4):392-408
pubmed: 13498658
Evol Appl. 2023 Mar 25;16(4):849-862
pubmed: 37124090
Nat Commun. 2016 Dec 06;7:13604
pubmed: 27922001
Evolution. 2022 Jan;76(1):86-100
pubmed: 34806781