FUS regulates the alternative splicing of cell proliferation genes related to atherosclerosis.
FUS
RNA-binding protein
RNA-seq
alternative splicing
atherosclerosis
Journal
Experimental biology and medicine (Maywood, N.J.)
ISSN: 1535-3699
Titre abrégé: Exp Biol Med (Maywood)
Pays: England
ID NLM: 100973463
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
pmc-release:
09
03
2024
medline:
27
11
2023
pubmed:
9
9
2023
entrez:
9
9
2023
Statut:
ppublish
Résumé
FUS plays a significant role as an RNA-binding protein in several cellular processes, including RNA splicing, DNA repair, and transcriptional regulation. However, the RNA-binding capacity of FUS in atherosclerosis is unclear. We aimed to study the functions of FUS in inflammatory regulation through the role of the splicing factor. We knocked down FUS with siRNA to further study the overall transcriptional level and select alternative splicing (AS) of FUS regulation in human umbilical vein endothelial cells (HUVECs) by RNA sequencing. The results suggested that the knockdown of FUS significantly affected gene expression in HUVECs. In addition, the knockdown of FUS resulted in 200 differentially expressed genes (DEGs) that were highly related to apoptotic process, signal transduction, multicellular organism development, cell adhesion and regulation of transcription, and DNA-templated pathways. Importantly, FUS extensively regulated 2870 AS events with a significant difference. Functional analysis of its modulated AS genes revealed they were highly enriched in cell cycle and cell population proliferation pathways. The qRT-PCR and RNA-seq data showed consistent results. Our findings suggested new knowledge of the mechanisms of FUS associated with atherosclerosis.
Identifiants
pubmed: 37688506
doi: 10.1177/15353702231187642
pmc: PMC10666725
doi:
Substances chimiques
RNA, Small Interfering
0
FUS protein, human
0
RNA-Binding Protein FUS
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1459-1468Déclaration de conflit d'intérêts
Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Références
Cell Metab. 2016 Dec 13;24(6):886
pubmed: 27974180
BMC Genomics. 2017 Feb 6;18(1):130
pubmed: 28166730
J Mol Cell Biol. 2009 Dec;1(2):82-92
pubmed: 19783543
Nat Med. 2010 Mar;16(3):279-85
pubmed: 20190767
J Lipid Res. 2014 Jun;55(6):1066-76
pubmed: 24729624
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
J Pathol. 2021 Feb;253(2):209-224
pubmed: 33125708
Atherosclerosis. 2015 Sep;242(1):145-54
pubmed: 26188538
J Reprod Immunol. 2007 Jun;74(1-2):34-45
pubmed: 17321596
Ann N Y Acad Sci. 2000 May;902:230-9; discussion 239-40
pubmed: 10865843
Nat Med. 2002 Aug;8(8):831-40
pubmed: 12091877
BMC Med. 2013 May 01;11:117
pubmed: 23635324
J Thromb Haemost. 2005 Mar;3(3):465-72
pubmed: 15748235
J Biol Chem. 2017 Jun 2;292(22):9283-9293
pubmed: 28389565
Signal Transduct Target Ther. 2019 Jul 19;4:23
pubmed: 31637003
J Mol Cell Cardiol. 2021 Nov;160:111-120
pubmed: 34302813
Nat Biotechnol. 2010 May;28(5):511-5
pubmed: 20436464
J Mol Cell Cardiol. 2021 Feb;151:106-112
pubmed: 33045252
Life Sci. 2018 Dec 1;214:153-157
pubmed: 30385177
Arterioscler Thromb Vasc Biol. 2008 Jun;28(6):1090-6
pubmed: 18388327
Oncol Rep. 2020 Jul;44(1):115-125
pubmed: 32377692
Scand J Clin Lab Invest. 2008;68(8):767-70
pubmed: 18622801
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Clin Invest. 2001 Oct;108(7):1031-40
pubmed: 11581304
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W316-22
pubmed: 21715386
Molecules. 2020 Jan 29;25(3):
pubmed: 32013236
Sci Rep. 2016 Oct 12;6:35195
pubmed: 27731383
Immunology. 2008 Feb;123(2):263-71
pubmed: 17725606
J Mol Diagn. 2000 Aug;2(3):132-8
pubmed: 11229517
Inflamm Allergy Drug Targets. 2009 Jul;8(3):208-20
pubmed: 19601881
EBioMedicine. 2017 Apr;18:188-198
pubmed: 28411140
Gene Ther. 2018 Dec;25(8):511-523
pubmed: 30287867
J Cell Physiol. 2019 Apr;234(4):5319-5326
pubmed: 30259979
Atherosclerosis. 2013 Aug;229(2):348-55
pubmed: 23880186
Circ Res. 1999 Jun 11;84(11):1258-67
pubmed: 10364563
Arch Cardiovasc Dis. 2016 Dec;109(12):708-715
pubmed: 27595467
Vascul Pharmacol. 2010 Jan-Feb;52(1-2):1-10
pubmed: 19878739
Blood. 2010 Nov 25;116(22):4646-56
pubmed: 20833976
Am J Pathol. 2004 Nov;165(5):1571-81
pubmed: 15509527
Nat Rev Mol Cell Biol. 2007 Jun;8(6):479-90
pubmed: 17473849
Cell Metab. 2012 Jun 6;15(6):861-72
pubmed: 22632897
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Front Cell Neurosci. 2016 Dec 26;10:290
pubmed: 28082870
Atherosclerosis. 2020 Apr;298:58-69
pubmed: 32171981
Ann Med. 2010;42(1):55-63
pubmed: 19941414
Cardiovasc Res. 2015 May 1;106(2):295-302
pubmed: 25750192
Circ Res. 2014 Jan 31;114(3):480-92
pubmed: 24281189
Atherosclerosis. 2012 Jun;222(2):355-9
pubmed: 22498256
Circulation. 2007 Oct 16;116(16):1812-20
pubmed: 17893273
J Proteome Res. 2018 Apr 6;17(4):1485-1499
pubmed: 29508616
J Mol Diagn. 2007 Sep;9(4):459-63
pubmed: 17620387
Clin Exp Immunol. 2007 Apr;148(1):106-11
pubmed: 17349013
Biochim Biophys Acta Gene Regul Mech. 2017 Sep;1860(9):911-921
pubmed: 28733224
J Am Coll Cardiol. 2006 Apr 18;47(8 Suppl):C7-12
pubmed: 16631513
Circ Res. 2004 Feb 6;94(2):253-61
pubmed: 14656931
J Biol Chem. 2011 Mar 4;286(9):7257-66
pubmed: 21190939
Cancer Res. 2003 Aug 1;63(15):4568-76
pubmed: 12907633
Vascul Pharmacol. 2015 Aug;71:57-64
pubmed: 25929188
Anal Biochem. 1987 Apr;162(1):156-9
pubmed: 2440339