E3 ubiquitin ligase APC/C

Cervical cancer Destabilization Post-translational modifications Ubiquitin-proteasome system Wound healing

Journal

Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Titre abrégé: Biochim Biophys Acta Gen Subj
Pays: Netherlands
ID NLM: 101731726

Informations de publication

Date de publication:
Nov 2023
Historique:
received: 15 06 2023
revised: 30 08 2023
accepted: 02 09 2023
pubmed: 10 9 2023
medline: 10 9 2023
entrez: 9 9 2023
Statut: ppublish

Résumé

The solute carrier family 35 F2 (SLC35F2), belongs to membrane-bound carrier proteins that control various physiological functions and are activated in several cancers. However, the molecular mechanism regulating SLC35F2 protein turnover and its implication in cancer progression remains unexplored. Therefore, screening for E3 ligases that promote SLC35F2 protein degradation is essential during cancer progression. The immunoprecipitation and Duolink proximity ligation assays (PLA) were used to determine the interaction between APC/C Based on the E3 ligase screen and in vitro biochemical experiments, we identified that APC/C Our study highlights that APC/C

Sections du résumé

BACKGROUND BACKGROUND
The solute carrier family 35 F2 (SLC35F2), belongs to membrane-bound carrier proteins that control various physiological functions and are activated in several cancers. However, the molecular mechanism regulating SLC35F2 protein turnover and its implication in cancer progression remains unexplored. Therefore, screening for E3 ligases that promote SLC35F2 protein degradation is essential during cancer progression.
METHODS METHODS
The immunoprecipitation and Duolink proximity ligation assays (PLA) were used to determine the interaction between APC/C
RESULTS RESULTS
Based on the E3 ligase screen and in vitro biochemical experiments, we identified that APC/C
CONCLUSION CONCLUSIONS
Our study highlights that APC/C

Identifiants

DOI: 10.1016/j.bbagen.2023.130454 PMID: 37689217
pubmed: 37689217
pii: S0304-4165(23)00152-6
doi: 10.1016/j.bbagen.2023.130454
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

130454

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors have no potential conflicts of interest to disclose.

Auteurs

Jencia Carminha Colaco (JC)

Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea.

Arun Pandian Chandrasekaran (AP)

Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea.

Janardhan Keshav Karapurkar (JK)

Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea.

D A Ayush Gowda (DAA)

Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea.

Girish Birappa (G)

Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea.

Sripriya Rajkumar (S)

Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea.

Bharathi Suresh (B)

Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea.

Nare Ko (N)

Biomedical Research Center, Asan Institute for Life Sciences, Seoul 05505, Republic of Korea; Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea.

Seok-Ho Hong (SH)

Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon, South Korea.

Seung Jun Oh (SJ)

Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. Electronic address: sjoh@amc.seoul.kr.

Kye-Seong Kim (KS)

Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea; College of Medicine, Hanyang University, Seoul, 04763, South Korea. Electronic address: ks66kim@hanyang.ac.kr.

Suresh Ramakrishna (S)

Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea; College of Medicine, Hanyang University, Seoul, 04763, South Korea. Electronic address: suri28@hanyang.ac.kr.

Classifications MeSH