Griddient: a microfluidic array to generate reconfigurable gradients on-demand for spatial biology applications.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
09 09 2023
09 09 2023
Historique:
received:
17
03
2023
accepted:
24
08
2023
medline:
11
9
2023
pubmed:
10
9
2023
entrez:
9
9
2023
Statut:
epublish
Résumé
Biological tissues are highly organized structures where spatial-temporal gradients (e.g., nutrients, hypoxia, cytokines) modulate multiple physiological and pathological processes including inflammation, tissue regeneration, embryogenesis, and cancer progression. Current in vitro technologies struggle to capture the complexity of these transient microenvironmental gradients, do not provide dynamic control over the gradient profile, are complex and poorly suited for high throughput applications. Therefore, we have designed Griddent, a user-friendly platform with the capability of generating controllable and reversible gradients in a 3D microenvironment. Our platform consists of an array of 32 microfluidic chambers connected to a 384 well-array through a diffusion port at the bottom of each reservoir well. The diffusion ports are optimized to ensure gradient stability and facilitate manual micropipette loading. This platform is compatible with molecular and functional spatial biology as well as optical and fluorescence microscopy. In this work, we have used this platform to study cancer progression.
Identifiants
pubmed: 37689746
doi: 10.1038/s42003-023-05282-3
pii: 10.1038/s42003-023-05282-3
pmc: PMC10492845
doi:
Substances chimiques
Cytokines
0
Banques de données
figshare
['10.6084/m9.figshare.2396101536', '10.6084/m9.figshare.2396103337', '10.6084/m9.figshare.2396103938', '10.6084/m9.figshare.2396104539', '10.6084/m9.figshare.2396105740']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
925Informations de copyright
© 2023. Springer Nature Limited.
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