Bioactive Suture with Added Innate Defense Functionality for the Reduction of Bacterial Infection and Inflammation.

TCP-25 host defense peptides polyglactin surgical site infections suture

Journal

Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613

Informations de publication

Date de publication:
09 Sep 2023
Historique:
revised: 04 09 2023
received: 28 03 2023
pubmed: 10 9 2023
medline: 10 9 2023
entrez: 10 9 2023
Statut: aheadofprint

Résumé

Surgical site infections (SSI) are a clinical and economic burden. Suture-associated SSI may develop when bacteria colonize the suture surface and form biofilms that are resistant to antibiotics. Thrombin-derived C-terminal peptide (TCP)-25 is a host defense peptide with a unique dual mode of action that can target both bacteria and the excessive inflammation induced by bacterial products. The peptide demonstrates therapeutic potential in preclinical in vivo wound infection models. In this study, the authors set out to explore whether TCP-25 can provide a new bioactive innate immune feature to hydrophilic polyglactin sutures (Vicryl). Using a combination of biochemical, biophysical, antibacterial, biofilm, and anti-inflammatory assays in vitro, in silico molecular modeling studies, along with experimental infection and inflammation models in mice, a proof-of-concept that TCP-25 can provide Vicryl sutures with a previously undisclosed host defense capacity, that enables targeting of bacteria, biofilms, and the accompanying inflammatory response, is shown.

Identifiants

pubmed: 37689972
doi: 10.1002/adhm.202300987
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2300987

Subventions

Organisme : Edvard Welanders Stiftelse and Finsenstiftelsen (HudFonden)
Organisme : Royal Physiographic Society
Organisme : Crafoord and Österlund Foundation
Organisme : Swedish Government Funds for Clinical Research (ALF)
Organisme : Swedish National Infrastructure for Biological Mass Spectrometry (BioMS)
Organisme : Alfred Österlunds Stiftelse
Organisme : Vetenskapsrådet
ID : 2017-02341,2020-02016
Organisme : Crafoordska Stiftelsen

Informations de copyright

© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.

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Auteurs

Manoj Puthia (M)

Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SE-22184, Sweden.

Jitka Petrlova (J)

Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SE-22184, Sweden.

Ganna Petruk (G)

Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SE-22184, Sweden.

Marta Butrym (M)

Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SE-22184, Sweden.

Firdaus Samsudin (F)

Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR), Singapore, 138671, Singapore.

Madelene Å Andersson (MÅ)

Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SE-22184, Sweden.

Ann-Charlotte Strömdahl (AC)

Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SE-22184, Sweden.

Sebastian Wasserstrom (S)

Lund University Bioimaging Centre, Lund University, Lund, SE-22184, Sweden.

Erik Hartman (E)

Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SE-22184, Sweden.

Sven Kjellström (S)

Division of Mass Spectrometry, Department of Clinical Sciences, Lund University, Lund, SE-22184, Sweden.

Lucrezia Caselli (L)

Physical Chemistry 1, Lund University, Lund, S-221 00, Sweden.

Oxana Klementieva (O)

Medical Microspectroscopy Lab, Department of Experimental Medical Sciences, Faculty of Medicine, Lund University, Lund, SE-221 84, Sweden.

Peter J Bond (PJ)

Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR), Singapore, 138671, Singapore.
Department of Biological Sciences, National University of Singapore, Singapore, 117543, Singapore.

Martin Malmsten (M)

Physical Chemistry 1, Lund University, Lund, S-221 00, Sweden.
Department of Pharmacy, University of Copenhagen, Copenhagen, DK-2100, Denmark.

Deepak Bushan Raina (DB)

Department of Clinical Sciences Lund, Orthopedics, Faculty of Medicine, Lund University, Lund, SE-221 84, Sweden.

Artur Schmidtchen (A)

Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, SE-22184, Sweden.
Dermatology, Skane University Hospital, Lund, SE-22185, Sweden.

Classifications MeSH