A mutant fitness compendium in Bifidobacteria reveals molecular determinants of colonization and host-microbe interactions.

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
29 Aug 2023
Historique:
pubmed: 11 9 2023
medline: 11 9 2023
entrez: 11 9 2023
Statut: epublish

Résumé

Bifidobacteria commonly represent a dominant constituent of human gut microbiomes during infancy, influencing nutrition, immune development, and resistance to infection. Despite interest as a probiotic therapy, predicting the nutritional requirements and health-promoting effects of Bifidobacteria is challenging due to major knowledge gaps. To overcome these deficiencies, we used large-scale genetics to create a compendium of mutant fitness in

Identifiants

DOI: 10.1101/2023.08.29.555234 PMID: 37693407 PMC: PMC10491234
pubmed: 37693407
doi: 10.1101/2023.08.29.555234
pmc: PMC10491234
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI147023
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM146949
Pays : United States
Organisme : NIGMS NIH HHS
ID : RM1 GM135102
Pays : United States

Auteurs

Anthony L Shiver (AL)

Department of Bioengineering, Stanford University, Stanford CA 94305, USA.

Jiawei Sun (J)

Department of Bioengineering, Stanford University, Stanford CA 94305, USA.

Rebecca Culver (R)

Department of Genetics, Stanford University, Stanford CA 94305, USA.

Arvie Violette (A)

Department of Bioengineering, Stanford University, Stanford CA 94305, USA.

Charles Wynter (C)

Department of Bioengineering, Stanford University, Stanford CA 94305, USA.

Marta Nieckarz (M)

Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, SE-90187, Sweden.

Samara Paula Mattiello (SP)

Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, 57007, USA.
College of Mathematics and Science, The University of Tennessee Southern, Pulaski TN 38478, USA.

Prabhjot Kaur Sekhon (PK)

Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, 57007, USA.
Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK, 74074, USA.

Lisa Friess (L)

School of Microbiology, University College Cork, Ireland.
APC Microbiome Ireland, University College Cork, Ireland.

Hans K Carlson (HK)

Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Daniel Wong (D)

Department of Applied Physics, Stanford University, Stanford CA 94305, USA.

Steven Higginbottom (S)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Meredith Weglarz (M)

Stanford Shared FACS Facility, Center for Molecular and Genetic Medicine, Stanford University, Stanford, California, USA.

Weigao Wang (W)

Department of Chemical Engineering, Stanford University, Stanford CA 94305, USA.

Benjamin D Knapp (BD)

Biophysics Program, Stanford University, Stanford, CA, USA.

Emma Guiberson (E)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Juan Sanchez (J)

Chan-Zuckerberg Biohub, San Francisco, CA 94158.

Po-Hsun Huang (PH)

Department of Mechanical Engineering, Laboratory for Energy and Microsystems Innovation, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139, MA, USA.

Paulo A Garcia (PA)

Department of Mechanical Engineering, Laboratory for Energy and Microsystems Innovation, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139, MA, USA.

Cullen R Buie (CR)

Department of Mechanical Engineering, Laboratory for Energy and Microsystems Innovation, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139, MA, USA.

Benjamin Good (B)

Department of Applied Physics, Stanford University, Stanford CA 94305, USA.

Brian DeFelice (B)

Chan-Zuckerberg Biohub, San Francisco, CA 94158.

Felipe Cava (F)

Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, SE-90187, Sweden.

Joy Scaria (J)

Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, 57007, USA.
Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK, 74074, USA.

Justin Sonnenburg (J)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Douwe Van Sinderen (DV)

School of Microbiology, University College Cork, Ireland.
APC Microbiome Ireland, University College Cork, Ireland.

Adam M Deutschbauer (AM)

Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.

Kerwyn Casey Huang (KC)

Department of Bioengineering, Stanford University, Stanford CA 94305, USA.
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.
Chan-Zuckerberg Biohub, San Francisco, CA 94158.

Classifications MeSH