Unravelling human hematopoietic progenitor cell diversity through association with intrinsic regulatory factors.
ATAC-seq
bone marrow
cord blood
erythrocyte/megakaryocyte lineage
fetal liver
hematopoietic stem and progenitor cell
human hematopoiesis
mass cytometry
mobilized peripheral blood
molecular regulator
myeloid lineage
pseudotime
single-cell
trajectory analysis
transcription factor
Journal
bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187
Informations de publication
Date de publication:
30 Aug 2023
30 Aug 2023
Historique:
pubmed:
11
9
2023
medline:
11
9
2023
entrez:
11
9
2023
Statut:
epublish
Résumé
Hematopoietic stem and progenitor cell (HSPC) transplantation is an essential therapy for hematological conditions, but finer definitions of human HSPC subsets with associated function could enable better tuning of grafts and more routine, lower-risk application. To deeply phenotype HSPCs, following a screen of 328 antigens, we quantified 41 surface proteins and functional regulators on millions of CD34+ and CD34- cells, spanning four primary human hematopoietic tissues: bone marrow, mobilized peripheral blood, cord blood, and fetal liver. We propose more granular definitions of HSPC subsets and provide new, detailed differentiation trajectories of erythroid and myeloid lineages. These aspects of our revised human hematopoietic model were validated with corresponding epigenetic analysis and
Identifiants
pubmed: 37693547
doi: 10.1101/2023.08.30.555623
pmc: PMC10491219
pii:
doi:
Types de publication
Preprint
Langues
eng
Subventions
Organisme : NIA NIH HHS
ID : P30 AG066515
Pays : United States
Organisme : NIBIB NIH HHS
ID : DP2 EB024246
Pays : United States
Organisme : NCI NIH HHS
ID : U24 CA224309
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG056287
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG057915
Pays : United States
Organisme : NIA NIH HHS
ID : U19 AG065156
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG068279
Pays : United States
Organisme : NHLBI NIH HHS
ID : U54 HL165445
Pays : United States
Déclaration de conflit d'intérêts
Declaration of interests I.S. is an employee of bluebird bio. The remaining authors declare no competing interests.
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