Performance upgrade of a microbial explosives' sensor strain by screening a high throughput saturation library of a transcriptional regulator.
Bioluminescence
Bioreporters
Escherichia coli
High-throughput screen (HTS)
Landmines
NGS
Precision Mutant Library
Protein engineering
Whole-cell biosensors
Journal
Computational and structural biotechnology journal
ISSN: 2001-0370
Titre abrégé: Comput Struct Biotechnol J
Pays: Netherlands
ID NLM: 101585369
Informations de publication
Date de publication:
2023
2023
Historique:
received:
31
05
2023
revised:
15
08
2023
accepted:
21
08
2023
medline:
13
9
2023
pubmed:
13
9
2023
entrez:
13
9
2023
Statut:
epublish
Résumé
We present a methodology for a high-throughput screening (HTS) of transcription factor libraries, based on bacterial cells and GFP fluorescence. The method is demonstrated on the
Identifiants
pubmed: 37701016
doi: 10.1016/j.csbj.2023.08.017
pii: S2001-0370(23)00292-1
pmc: PMC10493890
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4252-4260Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest related to our submitted manuscript “Performance upgrade of a microbial explosives’ sensor strain by screening a high throughput saturation library of a transcriptional regulator”.
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