Pharmacokinetics of delta-9-tetrahydrocannabinol following acute cannabis smoke exposure in mice; effects of sex, age, and strain.
11-COOH-Δ9THC
cannabis
pharmacokinetics
smoke
Δ9THC
Journal
Frontiers in pharmacology
ISSN: 1663-9812
Titre abrégé: Front Pharmacol
Pays: Switzerland
ID NLM: 101548923
Informations de publication
Date de publication:
2023
2023
Historique:
received:
22
05
2023
accepted:
08
08
2023
medline:
13
9
2023
pubmed:
13
9
2023
entrez:
13
9
2023
Statut:
epublish
Résumé
Increased use of cannabis and cannabinoids for recreational and medical purposes has led to a growth in research on their effects in animal models. The majority of this work has employed cannabinoid injections; however, smoking remains the most common route of cannabis consumption. To better model real-world cannabis use, we exposed mice to cannabis smoke to establish the pharmacokinetics of Δ9THC and its metabolites in plasma and brain. To determine the time course of Δ9THC and two major metabolites [11-hydroxy-delta-9-tetrahydrocannabinol (11-OH-THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (11-COOH-THC)], male and female C57BL/6J mice were exposed to smoke from sequentially burning 5 cannabis cigarettes. Following smoke exposure, trunk blood and brains were collected at 6 time points (10-240 min). Plasma and brain homogenates were analyzed for Δ9THC and metabolites using a validated ultraperformance liquid chromatography-tandem mass spectrometry method. To assess effects of age, sex, and mouse strain, we exposed mice of four strains (C57BL/6J, FVB, Swiss Webster, and 129S6/SvEv, aged 4-24 months) to cannabis using the same smoke regimen. Samples were collected 10 and 40 min following exposure. Lastly, to assess effects of dose, C57BL/6J mice were exposed to smoke from burning 3 or 5 cannabis cigarettes, with samples collected 40 min following exposure. The pharmacokinetic study revealed that maximum plasma Δ9THC concentrations (C
Identifiants
pubmed: 37701025
doi: 10.3389/fphar.2023.1227220
pii: 1227220
pmc: PMC10493391
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1227220Informations de copyright
Copyright © 2023 Gazarov, Zequeira, Senetra, Howard, Sharma, McCurdy, Lewis, Bizon and Setlow.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Appl Physiol (1985). 2005 Sep;99(3):807-13
pubmed: 15890760
Psychopharmacology (Berl). 2020 Feb;237(2):305-316
pubmed: 31637452
Anal Bioanal Chem. 2013 Dec;405(30):9791-803
pubmed: 24202191
Biochem Pharmacol. 1991 Apr 15;41(8):1187-94
pubmed: 1848985
Drug Alcohol Depend. 2013 Dec 1;133(2):763-7
pubmed: 24018317
Cannabis Cannabinoid Res. 2019 Dec 06;4(4):240-254
pubmed: 32042924
Curr Pharm Des. 2023 Apr 19;:
pubmed: 37114788
Mamm Genome. 2002 Aug;13(8):429-37
pubmed: 12226708
J Neurochem. 2004 Apr;89(1):134-41
pubmed: 15030397
Nat Genet. 2000 Aug;25(4):402-5
pubmed: 10932182
J Anal Toxicol. 2015 Oct;39(8):580-7
pubmed: 26378131
J Appl Physiol (1985). 2007 May;102(5):1927-35
pubmed: 17234796
Int J Biochem Cell Biol. 1995 Aug;27(8):741-6
pubmed: 7584607
Science. 2005 Jul 15;309(5733):476-81
pubmed: 16020737
Addict Sci Clin Pract. 2015 Apr 21;10:10
pubmed: 25896576
Br J Pharmacol. 2004 Mar;141(5):775-85
pubmed: 14757702
J Clin Pharmacol. 1981 Aug-Sep;21(S1):178S-189S
pubmed: 6271823
Am J Pathol. 2010 Jul;177(1):311-24
pubmed: 20522652
ERJ Open Res. 2021 Jul 19;7(3):
pubmed: 34291110
Acta Neuropathol. 2015 Nov;130(5):643-60
pubmed: 26197969
Eur Neuropsychopharmacol. 2017 Dec;27(12):1223-1237
pubmed: 29129557
PLoS One. 2016 Apr 11;11(4):e0153327
pubmed: 27065006
Mol Cell Neurosci. 2018 Oct;92:17-26
pubmed: 29859891
Genes Brain Behav. 2004 Jun;3(3):149-57
pubmed: 15140010
Life Sci. 1995;56(23-24):2089-95
pubmed: 7776836
J Anal Toxicol. 1992 Sep-Oct;16(5):276-82
pubmed: 1338215
Exp Gerontol. 2003 Aug;38(8):843-53
pubmed: 12915206
Addict Biol. 2022 Mar;27(2):e13125
pubmed: 34936167
Mol Pharmacol. 2011 Jun;79(6):964-73
pubmed: 21350020
Iran J Psychiatry. 2012 Fall;7(4):149-56
pubmed: 23408483
Clin Chem. 2016 Dec;62(12):1579-1592
pubmed: 27899456
J Anal Toxicol. 2017 Jul 1;41(6):508-516
pubmed: 28830124
J Anal Toxicol. 2017 May 1;41(4):277-288
pubmed: 28069869
J Neuroinflammation. 2012 Jan 16;9:8
pubmed: 22248049
Physiol Genomics. 2010 Oct;42A(2):103-13
pubmed: 20627938
Pharmacol Biochem Behav. 2006 Sep;85(1):105-13
pubmed: 16934319
Clin Pharmacokinet. 2003;42(4):327-60
pubmed: 12648025
Drug Alcohol Depend. 2001 Jul 1;63(2):107-16
pubmed: 11376914
Psychopharmacology (Berl). 2019 Sep;236(9):2773-2784
pubmed: 31044291
J Adolesc Health. 2019 Apr;64(4):487-493
pubmed: 30205931
Psychopharmacology (Berl). 2021 Dec;238(12):3595-3605
pubmed: 34495367
Chem Biodivers. 2007 Aug;4(8):1770-804
pubmed: 17712819
Sci Rep. 2021 Dec 14;11(1):23990
pubmed: 34907248
J Gen Intern Med. 2020 Jul;35(7):1979-1986
pubmed: 32291715
Acta Neuropathol Commun. 2018 Dec 19;6(1):137
pubmed: 30563574
J Anal Toxicol. 2008 Sep;32(7):470-7
pubmed: 18713514
Front Integr Neurosci. 2018 Oct 18;12:51
pubmed: 30405366
J Pharmacol Exp Ther. 2020 Jul;374(1):151-160
pubmed: 32345621
J Anal Toxicol. 2010 Oct;34(8):516-20
pubmed: 21258613
Clin Chem. 2015 Jun;61(6):850-69
pubmed: 26019183
Addict Biol. 2021 Jul;26(4):e12968
pubmed: 32985064
Neuropsychopharmacology. 2021 Apr;46(5):959-969
pubmed: 32927465
Psychopharmacology (Berl). 2014 Oct;231(20):3987-96
pubmed: 24696079
Addiction. 2009 Dec;104(12):2041-8
pubmed: 19804462
Value Health. 2019 Nov;22(11):1289-1294
pubmed: 31708066
Nature. 2002 Dec 5;420(6915):520-62
pubmed: 12466850