Natural human Bet v 1-specific IgG antibodies recognize non-conformational epitopes whereas IgE reacts with conformational epitopes.
Bet v 1
IgG
allergen
allergen-specific IgE
allergy
birch pollen allergy
epitope
Journal
Allergy
ISSN: 1398-9995
Titre abrégé: Allergy
Pays: Denmark
ID NLM: 7804028
Informations de publication
Date de publication:
12 2023
12 2023
Historique:
revised:
06
07
2023
received:
04
11
2022
accepted:
12
07
2023
medline:
4
12
2023
pubmed:
13
9
2023
entrez:
13
9
2023
Statut:
ppublish
Résumé
The nature of epitopes on Bet v 1 recognized by natural IgG antibodies of birch pollen allergic patients and birch pollen-exposed but non-sensitized subjects has not been studied in detail. To investigate IgE and IgG recognition of Bet v 1 and to study the effects of natural Bet v 1-specific IgG antibodies on IgE recognition of Bet v 1 and Bet v 1-induced basophil activation. Sera from birch pollen allergic patients (BPA, n = 76), allergic patients without birch pollen allergy (NBPA, n = 40) and non-allergic individuals (NA, n = 48) were tested for IgE, IgG as well as IgG IgE antibodies from BPA patients react almost exclusively with conformational epitopes whereas IgG, IgG IgE and IgG antibodies from BPA patients and birch pollen-exposed non-sensitized subjects recognize different epitopes. These findings explain why natural allergen-specific IgG do not protect against allergic symptoms and suggest that allergen-specific IgE and IgG have different clonal origin.
Sections du résumé
BACKGROUND
The nature of epitopes on Bet v 1 recognized by natural IgG antibodies of birch pollen allergic patients and birch pollen-exposed but non-sensitized subjects has not been studied in detail.
OBJECTIVE
To investigate IgE and IgG recognition of Bet v 1 and to study the effects of natural Bet v 1-specific IgG antibodies on IgE recognition of Bet v 1 and Bet v 1-induced basophil activation.
METHODS
Sera from birch pollen allergic patients (BPA, n = 76), allergic patients without birch pollen allergy (NBPA, n = 40) and non-allergic individuals (NA, n = 48) were tested for IgE, IgG as well as IgG
RESULTS
IgE antibodies from BPA patients react almost exclusively with conformational epitopes whereas IgG, IgG
CONCLUSION
IgE and IgG antibodies from BPA patients and birch pollen-exposed non-sensitized subjects recognize different epitopes. These findings explain why natural allergen-specific IgG do not protect against allergic symptoms and suggest that allergen-specific IgE and IgG have different clonal origin.
Substances chimiques
Epitopes
0
Antigens, Plant
0
Allergens
0
Immunoglobulin G
0
Immunoglobulin E
37341-29-0
Peptides
0
Plant Proteins
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3136-3153Subventions
Organisme : Megagrant
ID : 075-15-2021-632
Organisme : Austrian Science Fund
ID : P34472-B
Organisme : Danube ARC grant of the country of Lower Austria
Organisme : Russian Science Foundation
ID : 23-75-30016
Informations de copyright
© 2023 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.
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