A LAMP-based assay for the molecular detection of group B Streptococcus.

Pregnancy Infant, Newborn Humans Female Prospective Studies Streptococcus agalactiae / genetics Antibiotic Prophylaxis Hospitals
LAMP Neonatal infection Pregnant Screening Streptococcus agalactiae

Journal

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
ISSN: 1435-4373
Titre abrégé: Eur J Clin Microbiol Infect Dis
Pays: Germany
ID NLM: 8804297

Informations de publication

Date de publication:
Oct 2023
Historique:
received: 14 06 2023
accepted: 07 08 2023
medline: 25 9 2023
pubmed: 13 9 2023
entrez: 13 9 2023
Statut: ppublish

Résumé

Streptococcus agalactiae remains a major pathogen in human health, especially in neonatal infection. Detection in pregnant women is essential to initiate intrapartum antibiotic prophylaxis. This study compared the HiberGene loop-mediated isothermal amplification (LAMP) assay to culture, the reference method, for the detection of group B Streptococcus (GBS) in pregnant women. This was a prospective multicenter study conducted in four French hospitals. Three hundred fifty-four non-redundant routine care vaginal swabs were analyzed by both methods, LAMP assay and culture. Clinicians and patients were blinded to the results of the LAMP assay. Three hundred thirty-seven samples presented concordant results, 15 presented discordant results, and 2 were invalid using the LAMP assay (excluded from the study). Compared to culture, the LAMP assay had a sensitivity of 87.7%, a specificity of 98%, a negative predictive value of 97.6%, and a positive predictive value of 89.3%. The HiberGene GBS LAMP assay is an easy test that possesses good performances compared with the reference method, culture. It could be used in case of emergency when a quick result is needed.

Identifiants

DOI: 10.1007/s10096-023-04656-z PMID: 37702956
pubmed: 37702956
doi: 10.1007/s10096-023-04656-z
pii: 10.1007/s10096-023-04656-z
doi:

Types de publication

Multicenter Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1245-1250

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Références

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Auteurs

Coralie Lemaire (C)

Service de Bactériologie-Virologie, CHRU de Tours, Université de Tours, 37044, Tours, France.

Mélinda Cheminet (M)

Assistance Publique - Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Hôpital Cochin, Service de Bactériologie, Université Paris Cité, 75014, Paris, France.

Claire Duployez (C)

Laboratoire de Bactériologie, CHU de Lille, Université de Lille, 59000, Lille, France.

Mathilde Artus (M)

Laboratoire de Biologie Médicale, CH de Quimper, 29000, Quimper, France.

Yassine Ballaa (Y)

Assistance Publique - Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Hôpital Cochin, Service de Bactériologie, Université Paris Cité, 75014, Paris, France.

Laura Devos (L)

Laboratoire de Bactériologie, CHU de Lille, Université de Lille, 59000, Lille, France.

Céline Plainvert (C)

Assistance Publique - Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Hôpital Cochin, Service de Bactériologie, Université Paris Cité, 75014, Paris, France.
Centre National de Référence Des Streptocoques, Hôpital Cochin, 75014, Paris, France.

Claire Poyart (C)

Assistance Publique - Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Hôpital Cochin, Service de Bactériologie, Université Paris Cité, 75014, Paris, France.
Centre National de Référence Des Streptocoques, Hôpital Cochin, 75014, Paris, France.

Florence Le Gall (F)

Laboratoire de Biologie Médicale, CH de Quimper, 29000, Quimper, France.

Asmaa Tazi (A)

Assistance Publique - Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre, Hôpital Cochin, Service de Bactériologie, Université Paris Cité, 75014, Paris, France.
Centre National de Référence Des Streptocoques, Hôpital Cochin, 75014, Paris, France.

Philippe Lanotte (P)

Service de Bactériologie-Virologie, CHRU de Tours, Université de Tours, 37044, Tours, France. philippe.lanotte@univ-tours.fr.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Grossesse › A LAMP-based assay for the molecular detection...
questionsmedicales.fr › Personnes › Tranches d'âge › Nourrisson › Nouveau-né › A LAMP-based assay for the molecular detection...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A LAMP-based assay for the molecular detection...
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questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus agalactiae › A LAMP-based assay for the molecular detection...
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