Rapid detection of tear lactoferrin for diagnosis of dry eyes by using fluorescence polarization-based aptasensor.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 09 2023
13 09 2023
Historique:
received:
01
04
2023
accepted:
11
09
2023
medline:
15
9
2023
pubmed:
14
9
2023
entrez:
13
9
2023
Statut:
epublish
Résumé
Differentiating dry eye disease (DED) from allergic or viral conjunctivitis rapidly and accurately is important to ensure prompt diagnosis and treatment. Tear lactoferrin (LF), a multi-functional glycoprotein found in tears, decreases significantly in patients with DED, and has been considered as a DED diagnostic biomarker. Measuring tear LF level, however, takes time and requires the use of bulky instruments. Herein, a homogeneous carbon nanostructure-based aptasensor with high sensitivity and selectivity has been developed by applying fluorescence polarization (FP) technology. The FP of carbon dots (CDs) bioconjugated with LF aptamers (CDs-aptamer) is 21.2% higher than that of CDs, which can be further amplified (1.81 times) once interacting with graphene oxide nanosheets (GONS). In the presence of LF, GONS separates from CDs-aptamer because of the stronger binding affinity between CDs-aptamer to LF, resulting in the decrease of FP value. A linear relationship is observed between FP value and LF concentration in spiked tear samples from 0.66 to 3.32 mg/mL. The selectivity of the aptasensor has been investigated by measuring other proteins. The results indicate that the FP-based aptasensor is a cost-effective method with high sensitivity and selectivity in detection of tear LF.
Identifiants
pubmed: 37704755
doi: 10.1038/s41598-023-42484-5
pii: 10.1038/s41598-023-42484-5
pmc: PMC10499909
doi:
Substances chimiques
Lactoferrin
EC 3.4.21.-
graphene oxide
0
Carbon
7440-44-0
Oligonucleotides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15179Informations de copyright
© 2023. Springer Nature Limited.
Références
Findlay, Q. & Reid, K. Dry eye disease: When to treat and when to refer. Aust. Prescr. 41, 160 (2018).
pubmed: 30410213
pmcid: 6202299
Milner, M. S. et al. Dysfunctional tear syndrome: Dry eye disease and associated tear film disorders–new strategies for diagnosis and treatment. Curr. Opin. Ophthalmol. 28, 3 (2017).
pmcid: 5345890
Rouen, P. A. & White, M. L. Dry eye disease: Prevalence, assessment, and management. Home Healthc. Now 36, 74–83 (2018).
pubmed: 29498987
Vehof, J., Snieder, H., Jansonius, N. & Hammond, C. J. Prevalence and risk factors of dry eye in 79,866 participants of the population-based Lifelines cohort study in the Netherlands. Ocul. Surf. 19, 83–93 (2021).
pubmed: 32376389
Yu, J., Asche, C. V. & Fairchild, C. J. The economic burden of dry eye disease in the United States: A decision tree analysis. Cornea 30, 379–387 (2011).
pubmed: 21045640
Serin, D., Karsloglu, S., Kyan, A. & Alagöz, G. A simple approach to the repeatability of the Schirmer test without anesthesia: Eyes open or closed?. Cornea 26, 903–906 (2007).
pubmed: 17721285
Savini, G. et al. The challenge of dry eye diagnosis. Clin. Ophthalmol. 2, 31–55 (2008).
pubmed: 19668387
pmcid: 2698717
Flanagan, J. & Willcox, M. Role of lactoferrin in the tear film. Biochimie 91, 35–43 (2009).
pubmed: 18718499
Adlerova, L., Bartoskova, A. & Faldyna, M. Lactoferrin: A review. Veterinární Medicína 53, 457–468 (2008).
Ohashi, Y. et al. Abnormal protein profiles in tears with dry eye syndrome. Am. J. Ophthalmol. 136, 291–299 (2003).
pubmed: 12888052
Versura, P., Bavelloni, A., Grillini, M., Fresina, M. & Campos, E. C. Diagnostic performance of a tear protein panel in early dry eye. Mol. Vis. 19, 1247 (2013).
pubmed: 23761727
pmcid: 3675053
Ponzini, E., Scotti, L., Grandori, R., Tavazzi, S. & Zambon, A. Lactoferrin concentration in human tears and ocular diseases: A meta-analysis. Invest. Ophthalmol. Vis. Sci. 61, 9–9 (2020).
pubmed: 33035290
pmcid: 7552940
Wang, H.-F., Fukuda, M. & Shimomura, Y. Diagnosis of dry eye. Semin. Ophthalmol. 20, 53–62 (2005).
pubmed: 16020345
Zhang, Y., Lu, C. & Zhang, J. Lactoferrin and its detection methods: A review. Nutrients 13, 2492 (2021).
pubmed: 34444652
pmcid: 8398339
Smith, D. S. & Eremin, S. A. Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules. Anal. Bioanal. Chem. 391, 1499–1507 (2008).
pubmed: 18264817
Lakowicz, J. R. Principles of Fluorescence Spectroscopy 111–153 (Springer, 1983).
Hafner, M. et al. Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization. Nat. Protoc. 3, 579–587 (2008).
pubmed: 18388939
Lakowicz, J. R. Fluorescence Anisotropy. In Principles of fluorescence spectroscopy 3rd edn, (ed. Lakowicz, J. R.) 353–378 (Springer, New York, NY, 2006)
Gradinaru, C. C., Marushchak, D. O., Samim, M. & Krull, U. J. Fluorescence anisotropy: From single molecules to live cells. Analyst 135, 452–459 (2010).
pubmed: 20174695
Cui, L. et al. Mass amplifying probe for sensitive fluorescence anisotropy detection of small molecules in complex biological samples. Anal. Chem. 84, 5535–5541 (2012).
pubmed: 22686244
Tian, J. et al. Multiplexed detection of tumor markers with multicolor quantum dots based on fluorescence polarization immunoassay. Talanta 92, 72–77 (2012).
pubmed: 22385810
Ye, B. & Yin, B. Highly sensitive detection of mercury (II) ions by fluorescence polarization enhanced by gold nanoparticles. Angew. Chem. 120, 8514–8517 (2008).
Jiang, Y., Tian, J., Hu, K., Zhao, Y. & Zhao, S. Sensitive aptamer-based fluorescence polarization assay for mercury (II) ions and cysteine using silver nanoparticles as a signal amplifier. Microchim. Acta 181, 1423–1430 (2014).
Wang, L., Tian, J., Yang, W., Zhao, Y. & Zhao, S. A T7exonuclease-assisted target recycling amplification with graphene oxide acting as the signal amplifier for fluorescence polarization detection of human immunodeficiency virus (HIV) DNA. Luminescence 31, 573–579 (2016).
pubmed: 26935011
Roy, P., Chen, P.-C., Periasamy, A. P., Chen, Y.-N. & Chang, H.-T. Photoluminescent carbon nanodots: Synthesis, physicochemical properties and analytical applications. Mater. Today 18, 447–458 (2015).
Chen, Y., Qin, X., Yuan, C., Shi, R. & Wang, Y. Double responsive analysis of carbaryl pesticide based on carbon quantum dots and Au nanoparticles. Dyes Pigments 181, 108529 (2020).
Chen, L. Y., Dotzert, M., Melling, C. W. J. & Zhang, J. Tunable photoluminescence of carbon dots used for homogeneous glucose sensing assay. Biochem. Eng. J. 159, 07580 (2020).
Song, S., Wang, L., Li, J., Fan, C. & Zhao, J. Aptamer-based biosensors. TrAC Trends Anal. Chem. 27, 108–117 (2008).
Zhu, Z. et al. Single-stranded DNA binding protein-assisted fluorescence polarization aptamer assay for detection of small molecules. Anal. Chem. 84, 7203–7211 (2012).
pubmed: 22793528
Zhang, Y., Song, J., Yang, S., Ouyang, J. & Zhang, J. Carbon nanostructure-based DNA sensor used for quickly detecting breast cancer-associated genes. Nanoscale Res. Lett. 17, 1–9 (2022).
Liu, X., Li, H., Jia, W., Chen, Z. & Xu, D. Selection of aptamers based on a protein microarray integrated with a microfluidic chip. Lab. Chip 17, 178–185 (2017).
Zhang, Y. Q., Tang, H., Chen, W. & Zhang, J. Nanomaterials used in fluorescence polarization based biosensors. Int. J. Mol. Sci. 23(15), 24–30 (2022).
Wang, H. & Ceulemans, A. Physisorption of adenine DNA nucleosides on zigzag and armchair single-walled carbon nanotubes: a first-principles study. Phys. Rev. B 79, 195419–195422 (2009).
Zeng, S. W., Chen, L., Wang, Y. & Chen, J. L. Exploration on the mechanism of DNA adsorption on graphene and graphene oxide via molecular simulations. J. Phys. D Appl. Phys. 48, 275402 (2015).
Zhang, Y., Song, J., Yang, S., Ouyang, J. Y. & Zhang, J. Carbon nanostructure-based DNA sensor used for quickly detecting breast cancer-associated genes. Nanoscale Res. Lett. 17, 93 (2022).
pubmed: 36125561
pmcid: 9489825
Chen, Z. et al. Bivalent aptasensor based on silver-enhanced fluorescence polarization for rapid detection of lactoferrin in milk. Anal. Chem. 89, 5900–5908 (2017).
pubmed: 28467701
Chen, L. Y., Dotzert, M., Melling, C. W. J. & Zhang, J. Tunable photoluminescence of carbon dots used for homogeneous glucose sensing assay. Biochem. Eng. J. 159, 107580 (2020).
Song, J. & Zhang, J. Self-illumination of carbon dots by bioluminescence resonance energy transfer. Sci. Rep. 9, 13796–14137 (2019).
pubmed: 31551471
pmcid: 6760201
Zhang, Y. P. & Zhang, J. Fluorescence resonance energy transfer-based aptasensor made of carbon-based nanomaterials for detecting lactoferrin at low concentrations. ACS Omega 7(42), 37964–37970 (2022).
pubmed: 36312380
pmcid: 9609055
Abdolhosseinzadeh, S., Asgharzadeh, H. & Seop Kim, H. Fast and fully-scalable synthesis of reduced graphene oxide. Sci. Rep. 5, 1–7 (2015).
Jameson, D. M. & Ross, J. A. Fluorescence polarization/anisotropy in diagnostics and imaging. Chem. Rev. 110, 2685–2708 (2010).
pubmed: 20232898
pmcid: 2868933
Tan, M. et al. Enhanced photoluminescence and characterization of multicolor carbon dots using plant soot as a carbon source. Talanta 115, 950–956 (2013).
pubmed: 24054687
Zhao, L. et al. Chemiluminescence of carbon dots under strong alkaline solutions: A novel insight into carbon dot optical properties. Nanoscale 5, 2655–2658 (2013).
pubmed: 23456202
Haris, P. I. & Chapman, D. The conformational analysis of peptides using Fourier transform IR spectroscopy. Biopolym. Orig. Res. Biomol. 37, 251–263 (1995).
Bourlinos, A. B. et al. Photoluminescent carbogenic dots. Chem. Mater. 20(14), 4539–4541 (2008).
Lai, Q., Zhu, S. F., Luo, X. P., Zou, M. & Huang, S. H. Ultraviolet-visible spectroscopy of graphene oxides. AIP Adv. 2, 032146 (2012).
Fujishima, H., Toda, I., Shimazaki, J. & Tsubota, K. Allergic conjunctivitis and dry eye. Br. J. Ophthalmol. 80, 994–997 (1996).
pubmed: 8976728
pmcid: 505678