Community-developed checklists for publishing images and image analyses.


Journal

Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604

Informations de publication

Date de publication:
Feb 2024
Historique:
received: 17 02 2023
accepted: 26 07 2023
pmc-release: 01 02 2025
medline: 15 2 2024
pubmed: 15 9 2023
entrez: 14 9 2023
Statut: ppublish

Résumé

Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.

Identifiants

pubmed: 37710020
doi: 10.1038/s41592-023-01987-9
pii: 10.1038/s41592-023-01987-9
pmc: PMC10922596
mid: NIHMS1934198
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

170-181

Subventions

Organisme : Medical Research Council
ID : MR/W020793/1
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : P41 GM135019
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA200059
Pays : United States

Commentaires et corrections

Type : UpdateOf

Informations de copyright

© 2023. Springer Nature America, Inc.

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Auteurs

Christopher Schmied (C)

Fondazione Human Technopole, Milano, Italy. schmied@fmp-berlin.de.
Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany. schmied@fmp-berlin.de.

Michael S Nelson (MS)

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.

Sergiy Avilov (S)

Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany.

Gert-Jan Bakker (GJ)

Medical BioSciences Department, Radboud University Medical Centre, Nijmegen, the Netherlands.

Cristina Bertocchi (C)

Laboratory for Molecular Mechanics of Cell Adhesions, Pontificia Universidad Católica de Chile Santiago, Santiago de Chile, Chile.
Graduate School of Engineering Science, Osaka University, Osaka, Japan.

Johanna Bischof (J)

Euro-BioImaging ERIC, Bio-Hub, Heidelberg, Germany.

Ulrike Boehm (U)

Carl Zeiss AG, Oberkochen, Germany.

Jan Brocher (J)

Scientific Image Processing and Analysis, BioVoxxel, Ludwigshafen, Germany.

Mariana T Carvalho (MT)

Nanophotonics and BioImaging Facility at INL, International Iberian Nanotechnology Laboratory, Braga, Portugal.

Catalin Chiritescu (C)

Phi Optics, Inc., Champaign, IL, USA.

Jana Christopher (J)

Biochemistry Center Heidelberg, Heidelberg University, Heidelberg, Germany.

Beth A Cimini (BA)

Imaging Platform, Broad Institute, Cambridge, MA, USA.

Eduardo Conde-Sousa (E)

i3S, Instituto de Investigação e Inovação Em Saúde and INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.

Michael Ebner (M)

Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.

Rupert Ecker (R)

Translational Research Institute, Queensland University of Technology, Woolloongabba, Queensland, Australia.
School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia.
TissueGnostics GmbH, Vienna, Austria.

Kevin Eliceiri (K)

Department of Medical Physics and Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.

Julia Fernandez-Rodriguez (J)

Centre for Cellular Imaging Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Nathalie Gaudreault (N)

Allen Institute for Cell Science, Seattle, WA, USA.

Laurent Gelman (L)

Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

David Grunwald (D)

RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA.

Tingting Gu (T)

University of Oklahoma, Norman, OK, USA.

Nadia Halidi (N)

Advanced Light Microscopy Unit, Centre for Genomic Regulation, Barcelona, Spain.

Mathias Hammer (M)

RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA.

Matthew Hartley (M)

European Molecular Biology Laboratory (EMBL), European Bioinformatics Institute, Hinxton, UK.

Marie Held (M)

Centre for Cell Imaging, the University of Liverpool, Liverpool, UK.

Florian Jug (F)

Fondazione Human Technopole, Milano, Italy.

Varun Kapoor (V)

Department of AI Research, Kapoor Labs, Paris, France.

Ayse Aslihan Koksoy (AA)

MD Anderson Cancer Center, Houston, TX, USA.

Judith Lacoste (J)

MIA Cellavie Inc., Montreal, Quebec, Canada.

Sylvia Le Dévédec (S)

Division of Drug Discovery and Safety, Cell Observatory, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands.

Sylvie Le Guyader (S)

Karolinska Institutet, Huddinge, Sweden.

Penghuan Liu (P)

Key Laboratory for Modern Measurement Technology and Instruments of Zhejiang Province, College of Optical and Electronic Technology, China Jiliang University, Hangzhou, China.

Gabriel G Martins (GG)

Advanced Imaging Facility, Instituto Gulbenkian de Ciência, Oeiras, Portugal.

Aastha Mathur (A)

Euro-BioImaging ERIC, Bio-Hub, Heidelberg, Germany.

Kota Miura (K)

Bioimage Analysis and Research, Heidelberg, Germany.

Paula Montero Llopis (P)

MicRoN Core, Harvard Medical School, Boston, MA, USA.

Roland Nitschke (R)

Life Imaging Center, Signalling Research Centres CIBSS and BIOSS, University of Freiburg, Freiburg, Germany.

Alison North (A)

Bio-Imaging Resource Center, the Rockefeller University, New York, NY, USA.

Adam C Parslow (AC)

Baker Institute Microscopy Platform, Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia.

Alex Payne-Dwyer (A)

School of Physics, Engineering and Technology, University of York, Heslington, UK.

Laure Plantard (L)

Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Rizwan Ali (R)

King Abdullah International Medical Research Center (KAIMRC), Medical Research Core Facility and Platforms (MRCFP), King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.

Britta Schroth-Diez (B)

Light Microscopy Facility, Max Planck Institute of Molecular Cell Biology and Genetics Dresden, Dresden, Germany.

Lucas Schütz (L)

ariadne.ai (Germany) GmbH, Heidelberg, Germany.

Ryan T Scott (RT)

Space Biosciences Division, NASA Ames Research Center, Moffett Field, CA, USA.

Arne Seitz (A)

BioImaging and Optics Platform, Faculty of Life Sciences (SV), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Olaf Selchow (O)

Microscopy and BioImaging Consulting, Image Processing and Large Data Handling, Gera, Germany.

Ved P Sharma (VP)

Bio-Imaging Resource Center, the Rockefeller University, New York, NY, USA.

Martin Spitaler (M)

Max Planck Institute of Biochemistry, Martinsried, Germany.

Sathya Srinivasan (S)

Imaging and Morphology Support Core, Oregon National Primate Research Center, OHSU West Campus, Beaverton, OR, USA.

Caterina Strambio-De-Castillia (C)

Program in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA.

Douglas Taatjes (D)

Department of Pathology and Laboratory Medicine, Microscopy Imaging Center, Center for Biomedical Shared Resources, University of Vermont, Burlington, VT, USA.

Christian Tischer (C)

Centre for Bioimage Analysis, EMBL Heidelberg, Heidelberg, Germany. christian.tischer@embl.de.

Helena Klara Jambor (HK)

NCT-UCC, Medizinische Fakultät TU Dresden, Dresden, Germany. helena.jambor@tu-dresden.de.

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