Development of an Efficient Recombinant Protein Expression System in

Bacteriophage T7 / genetics Lactose Recombinant Proteins / metabolism DNA-Directed RNA Polymerases / genetics Clostridium / genetics
Clostridium T7 expression system anaerobic endotoxin-free inducible expression recombinant proteins

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
20 10 2023
Historique:
medline: 23 10 2023
pubmed: 15 9 2023
entrez: 15 9 2023
Statut: ppublish

Résumé

Recombinant proteins have broad applications. However, there is a lack of a recombinant protein expression system specifically for large-scale production in anaerobic hosts. Here, we developed a powerful and stringently inducible protein expression system based on the bacteriophage T7 system in the strictly anaerobic solvent-producing

Identifiants

DOI: 10.1021/acssynbio.3c00439 PMID: 37712503
pubmed: 37712503
doi: 10.1021/acssynbio.3c00439
doi:

Substances chimiques

Lactose J2B2A4N98G
Recombinant Proteins 0
DNA-Directed RNA Polymerases EC 2.7.7.6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3092-3105

Auteurs

Yuechao Ma (Y)

Department of Biosystems Engineering, Auburn University, Auburn, Alabama 36849, United States.
Center for Bioenergy and Bioproducts, Auburn University, Auburn, Alabama 36849, United States.

Na Guo (N)

Department of Biosystems Engineering, Auburn University, Auburn, Alabama 36849, United States.
Center for Bioenergy and Bioproducts, Auburn University, Auburn, Alabama 36849, United States.

Xiao Li (X)

Department of Crop, Soil and Environmental Sciences, Auburn University, Auburn, Alabama 36849, United States.

Zhihua Jiang (Z)

Department of Chemical Engineering, Auburn University, Auburn, Alabama 36849, United States.

Dunhua Zhang (D)

Aquatic Animal Health Research Unit, Agricultural Research Service, USDA, Auburn, Alabama 36832, United States.

Liang Guo (L)

College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
ORCID: 0000-0002-2481-7256

Yi Wang (Y)

Department of Biosystems Engineering, Auburn University, Auburn, Alabama 36849, United States.
Center for Bioenergy and Bioproducts, Auburn University, Auburn, Alabama 36849, United States.
ORCID: 0000-0002-0192-3195

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Classifications MeSH

questionsmedicales.fr › Virus › Virus à ADN › Caudovirales › Podoviridae › Bactériophage T7 › Development of an Efficient Recombinant...
questionsmedicales.fr › Glucides › Sucres › Diholoside › Lactose › Development of an Efficient Recombinant...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Development of an Efficient Recombinant...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › Development of an Efficient Recombinant...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bactéries à Gram positif formant des endospores › Clostridium › Development of an Efficient Recombinant...