Fluorescence Spectroscopy Based Characterization of Pseudomonas Aeruginosa Suspension.

Colony forming unit (CFU) Intrinsic tryptophan NADH and FAD fluorescence Optical density method Plate count method Pseudomonas aeruginosa tyrosine

Journal

Journal of fluorescence

ISSN: 1573-4994

Titre abrégé: J Fluoresc

Pays: Netherlands

ID NLM: 9201341

Informations de publication

Date de publication:
15 Sep 2023

Historique:

received: 16 07 2023

accepted: 06 09 2023

medline: 15 9 2023

pubmed: 15 9 2023

entrez: 15 9 2023

Statut: aheadofprint

Résumé

In this article, optical characterization of Pseudomonas aeruginosa (PA) suspension has been performed by using Fluorescence spectroscopy. Optical density (OD) and plate count methods have been employed as a reference for the analysis of emission spectra of Pseudomonas aeruginosa in water suspension. Emission spectra of PA suspension has been acquired by using excitation wavelengths from 270 to 420 nm with step of 10 nm to explore its spectral behavior. It has been found that emission spectra of tryptophan, tyrosine, NADH and FAD, the intracellular biomolecules of bacteria, can be used as finger prints for the detection of Pseudomonas aeruginosa. Furthermore, the effect of water matrix on the spectral emission of Pseudomonas aeruginosa has been investigated that might be one of the limitation of Fluorescence spectroscopy for complex water matrices. Moreover, a calibration curve has been produced between ODs

Identifiants

DOI: 10.1007/s10895-023-03436-4 PMID: 37713016

pubmed: 37713016

doi: 10.1007/s10895-023-03436-4

pii: 10.1007/s10895-023-03436-4

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Subventions

Organisme : Higher Education Commision, Pakistan

ID : 20-14617/NRPU/R&D/HEC/2021 2021

Organisme : Higher Education Commision, Pakistan

ID : 20-14617/NRPU/R&D/HEC/2021 2021

Informations de copyright

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