A Millifluidic Chamber for Controlled Shear Stress Testing: Application to Microbial Cultures.
Cell adhesion
Computational fluid dynamics
Flow-exposed cell cultures
Human gut microbiota
In vitro models
Journal
Annals of biomedical engineering
ISSN: 1573-9686
Titre abrégé: Ann Biomed Eng
Pays: United States
ID NLM: 0361512
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
24
04
2023
accepted:
03
09
2023
medline:
9
11
2023
pubmed:
15
9
2023
entrez:
15
9
2023
Statut:
ppublish
Résumé
In vitro platforms such as bioreactors and microfluidic devices are commonly designed to engineer tissue models as well as to replicate the crosstalk between cells and microorganisms hosted in the human body. These systems promote nutrient supply and waste removal through culture medium recirculation; consequently, they intrinsically expose cellular structures to shear stress, be it a desired mechanical stimulus to drive the cell fate or a potential inhibitor for the model maturation. Assessing the impact of shear stress on cellular or microbial cultures thus represents a crucial step to define proper environmental conditions for in vitro models. In this light, the aim of this study was to develop a millifluidic device enabling to generate fully controlled shear stress profiles for quantitatively probing its influence on tissue or bacterial models, overcoming the limitations of previous reports proposing similar devices. Relying on this millifluidic tool, we present a systematic methodology to test how adherent cellular structures react to shear forces, which was applied to the case of microbial biofilms as a proof of concept. The results obtained suggest our approach as a suitable testbench to evaluate culture conditions in terms of shear stress faced by cells or microorganisms.
Identifiants
pubmed: 37713099
doi: 10.1007/s10439-023-03361-4
pii: 10.1007/s10439-023-03361-4
pmc: PMC10632311
doi:
Substances chimiques
Culture Media
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2923-2933Informations de copyright
© 2023. The Author(s).
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