An overview of SARS-CoV-2 variants circulating in the 2020-2022 period in Lombardy.


Journal

Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899

Informations de publication

Date de publication:
Dec 2023
Historique:
received: 19 05 2023
revised: 21 08 2023
accepted: 22 08 2023
medline: 13 11 2023
pubmed: 16 9 2023
entrez: 15 9 2023
Statut: ppublish

Résumé

Since the beginning of the pandemic, SARS-CoV-2 has shown genetic variability. All the variants that have sustained pandemic waves have shown several mutations, especially in the Spike protein that could affect viral pathogenesis. A total of 15,729 respiratory samples, collected between December 2020 and August 2022, have been included in this study. We report the circulation of SARS-CoV-2 variants in the Lombardy region, Italy, in a 2-year study period. Alpha, Delta, and Omicron variants became predominant causing the majority of cases whereas Beta or Gamma variants mostly caused local outbreaks. Next-generation sequencing revealed several mutations and few deletions in all of the main variants. For example, 147 mutations were observed in the Spike protein of Omicron sublineages; 20% of these mutations occurred in the receptor-binding domain region.

Identifiants

pubmed: 37714081
pii: S0732-8893(23)00179-7
doi: 10.1016/j.diagmicrobio.2023.116070
pii:
doi:

Substances chimiques

Spike Glycoprotein, Coronavirus 0
spike protein, SARS-CoV-2 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

116070

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Federica Giardina (F)

Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy.

Guglielmo Ferrari (G)

Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Federica Zavaglio (F)

Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Stefania Paolucci (S)

Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Francesca Rovida (F)

Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Giulia Campanini (G)

Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Laura Pellegrinelli (L)

Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.

Cristina Galli (C)

Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.

Elena Pariani (E)

Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.

Federica Bergami (F)

Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Alice Nava (A)

Microbiological Analysis Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.

Elisa Matarazzo (E)

Microbiological Analysis Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.

Silvia Renica (S)

Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy.

Diana Fanti (D)

Microbiological Analysis Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.

Valeria Cento (V)

Department of Biomedical Sciences, Humanitas University, Milan, Italy; IRCCS Humanitas Research Hospital, Milan, Italy.

Claudia Alteri (C)

Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy.

Francesco Scaglione (F)

Department of Oncology and Hemato-oncology, University of Milan, Milan, Italy.

Chiara Vismara (C)

Microbiological Analysis Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.

Carlo Federico Perno (CF)

Multimodal Research Area, Unit of Microbiology and Diagnostic Immunology, Bambino Gesù Children Hospital IRCCS, Rome, Italy.

Antonio Piralla (A)

Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. Electronic address: a.piralla@smatteo.pv.it.

Fausto Baldanti (F)

Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

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Classifications MeSH