An electrochemical biosensor for T4 polynucleotide kinase activity identification according to host-guest recognition among phosphate pillar[5]arene@palladium nanoparticles@reduced graphene oxide nanocomposite and toluidine blue.

Electrochemical biosensors Gold electrode modification  Host–guest recognition Palladium nanoparticles Phosphate pillar[5]arene T4 polynucleotide kinase Toluidine blue

Journal

Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782

Informations de publication

Date de publication:
16 09 2023
Historique:
received: 11 06 2023
accepted: 02 09 2023
medline: 18 9 2023
pubmed: 16 9 2023
entrez: 15 9 2023
Statut: epublish

Résumé

T4 polynucleotide kinase (T4 PNK) helps with DNA recombination and repair. In this work, a phosphate pillar[5]arene@palladium nanoparticles@reduced graphene oxide nanocomposite (PP5@PdNPs@rGO)-based electrochemical biosensor was created to identify T4 PNK activities. The PP5 used to complex toluidine blue (TB) guest molecules is water-soluble. With T4 PNK and ATP, the substrate DNA, which included a 5'-hydroxyl group, initially self-assembled over the gold electrode surface by chemical adsorption of the thiol units. Strong phosphate-Zr

Identifiants

pubmed: 37715009
doi: 10.1007/s00604-023-05983-w
pii: 10.1007/s00604-023-05983-w
doi:

Substances chimiques

graphene oxide 0
palladium oxide B30901Q32J
Palladium 5TWQ1V240M
Phosphates 0
pillar(5)arene 0
Polynucleotide 5'-Hydroxyl-Kinase EC 2.7.1.78
Tolonium Chloride 15XUH0X66N

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

394

Subventions

Organisme : National Natural Science Foundation of China
ID : 21665027
Organisme : National Natural Science Foundation of China
ID : 21565031

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

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Auteurs

Yongju Wu (Y)

Key Laboratory of Environmental Functional Materials of Yunnan Province Education Department, Yunnan Minzu University, Kunming, 650504, People's Republic of China.

Jinfei Yi (J)

Key Laboratory of Environmental Functional Materials of Yunnan Province Education Department, Yunnan Minzu University, Kunming, 650504, People's Republic of China.

Aiwen Su (A)

Key Laboratory of Environmental Functional Materials of Yunnan Province Education Department, Yunnan Minzu University, Kunming, 650504, People's Republic of China.

Yanli Zhang (Y)

Key Laboratory of Environmental Functional Materials of Yunnan Province Education Department, Yunnan Minzu University, Kunming, 650504, People's Republic of China. ylzhang@ymu.edu.cn.

Hongbin Wang (H)

Key Laboratory of Environmental Functional Materials of Yunnan Province Education Department, Yunnan Minzu University, Kunming, 650504, People's Republic of China.

Lijuan Yang (L)

Key Laboratory of Environmental Functional Materials of Yunnan Province Education Department, Yunnan Minzu University, Kunming, 650504, People's Republic of China.

Wenrong Yang (W)

School of Life and Environmental Sciences, Deakin University, Geelong, VIC, 3217, Australia.

Pengfei Pang (P)

Key Laboratory of Environmental Functional Materials of Yunnan Province Education Department, Yunnan Minzu University, Kunming, 650504, People's Republic of China.

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