Type 2 T-Cell Responses against Distinct Epitopes of the Desmoglein 3 Ectodomain in Pemphigus Vulgaris.


Journal

The Journal of investigative dermatology
ISSN: 1523-1747
Titre abrégé: J Invest Dermatol
Pays: United States
ID NLM: 0426720

Informations de publication

Date de publication:
16 Sep 2023
Historique:
received: 08 11 2022
revised: 26 06 2023
accepted: 03 07 2023
pubmed: 18 9 2023
medline: 18 9 2023
entrez: 17 9 2023
Statut: aheadofprint

Résumé

Pemphigus vulgaris (PV) is an autoimmune blistering disorder of the skin and/or mucous membranes caused by IgG autoantibodies that predominantly target two transmembrane desmosomal cadherins: desmoglein (DSG)1 and DSG3. DSG-specific T cells play a central role in PV pathogenesis because they provide help to autoreactive B cells for autoantibody production. In this study, we characterized DSG3-specific peripheral T cells in a cohort of 52 patients with PV and 41 healthy controls with regard to cytokine profile and epitope specificity. By ELISpot analysis, type 2 T cells reactive with the DSG3 ectodomain were significantly increased in patients with PV compared with those in healthy controls. By dextramer analysis, CD4+ T cells specific for an epitope within the extracellular domain of DSG3, DSG3

Identifiants

pubmed: 37717934
pii: S0022-202X(23)02576-9
doi: 10.1016/j.jid.2023.07.025
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Dario Didona (D)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Luca Scarsella (L)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Christoph Hudemann (C)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Karolin Volkmann (K)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Christine L Zimmer (CL)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Benedikt Beckert (B)

Institute of Biochemistry, Medical Faculty, University of Giessen, Germany.

Ritva Tikkanen (R)

Institute of Biochemistry, Medical Faculty, University of Giessen, Germany.

Vera Korff (V)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Katja Kühn (K)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Sandra Wienzek-Lischka (S)

Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany.

Gregor Bein (G)

Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany.

Giovanni Di Zenzo (G)

Laboratory of Molecular and Cell Biology, Istituto Dermopatico dell'Immacolata (IDI), Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy.

Jaqueline Böhme (J)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Tomas Cunha (T)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Farzan Solimani (F)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany; Department of Dermatology, Venereology and Allergology, Charité-Universitätsmedizin Berlin, Berlin, Germany; Berlin Institute of Health at Charité-Universitätsmedizin Berlin, BIH Biomedical Innovation Academy, Berlin, Germany.

Josquin Pieper (J)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Hazem A Juratli (HA)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany; Department of Dermatology, University Hospital Basel, Basel, Switzerland.

Manuel Göbel (M)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Thomas Schmidt (T)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany.

Luca Borradori (L)

Department of Dermatology, University of Bern, Bern, Switzerland.

Amir S Yazdi (AS)

Department of Dermatology, RWTH Aachen University, Aachen, Germany.

Cassian Sitaru (C)

Department of Dermatology, Albert-Ludwigs University, Freiburg, Germany.

Holger Garn (H)

Translational Inflammation Research Division & Core Facility for Single Cell Multiomics, Philipps University, Marburg, Germany.

Rüdiger Eming (R)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany; Klinik III Dermatologie, Venerologie & Allergologie, Bundeswehrzentralkrankenhaus Koblenz, Koblenz, Germany.

Sabine Fleischer (S)

Topas Therapeutics, Hamburg, Germany.

Michael Hertl (M)

Department of Dermatology and Allergology, Philipps University, Marburg, Germany. Electronic address: hertl@uni-marburg.de.

Classifications MeSH