Effects of methylation and imprinting expression of Insulin-like growth factor 2 gene in gastric cancer.

Humans Pregnancy Female DNA Methylation Genomic Imprinting Stomach Neoplasms / genetics Epstein-Barr Virus Infections / complications Herpesvirus 4, Human / genetics Somatomedins / genetics
DNA methylation Epstein-barr virus gastric cancer genomic imprinting insulin-like growth factor 2

Journal

Cancer biomarkers : section A of Disease markers
ISSN: 1875-8592
Titre abrégé: Cancer Biomark
Pays: Netherlands
ID NLM: 101256509

Informations de publication

Date de publication:
2023
Historique:
medline: 27 11 2023
pubmed: 18 9 2023
entrez: 18 9 2023
Statut: ppublish

Résumé

Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC) is a common malignant tumor associated with EBV infection. Insulin-like growth factor 2 (IGF2) is an imprinted gene and a key protein that regulates growth, especially during normal fetal development. Loss of imprinting (LOI), is a common epigenetic anomaly in a variety of human cancers. However, the promoter methylation, imprinting status and function of IGF2 gene in GC are unclear. To explore the role of IGF2 in the occurrence and development of gastric cancer. The biological function of IGF2 in gastric cancer was investigated by Transwell, wound healing, CCK-8 and flow cytometry assays. IGF2 imprinting status and gene promoter methylation in gastric cancer tissues were detected by PCR-RFLP and BGS. The results showed that the expression of IGF2 was higher in GC tissues than adjacent tissues. IGF2 gene promoter methylation and LOI were significantly higher in EBVaGC tissues than in EBV-negative gastric cancer (EBVnGC) tissues. The high expression of IGF2 in gastric cancer can promote the migration and proliferation of gastric cancer cells. Our data suggest that IGF2 is involved in the occurrence and development of gastric cancer. Targeting IGF2 may be a potential therapeutic target for gastric cancer.

Sections du résumé

BACKGROUND BACKGROUND
Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC) is a common malignant tumor associated with EBV infection. Insulin-like growth factor 2 (IGF2) is an imprinted gene and a key protein that regulates growth, especially during normal fetal development. Loss of imprinting (LOI), is a common epigenetic anomaly in a variety of human cancers. However, the promoter methylation, imprinting status and function of IGF2 gene in GC are unclear.
OBJECTIVE OBJECTIVE
To explore the role of IGF2 in the occurrence and development of gastric cancer.
METHODS METHODS
The biological function of IGF2 in gastric cancer was investigated by Transwell, wound healing, CCK-8 and flow cytometry assays. IGF2 imprinting status and gene promoter methylation in gastric cancer tissues were detected by PCR-RFLP and BGS.
RESULTS RESULTS
The results showed that the expression of IGF2 was higher in GC tissues than adjacent tissues. IGF2 gene promoter methylation and LOI were significantly higher in EBVaGC tissues than in EBV-negative gastric cancer (EBVnGC) tissues. The high expression of IGF2 in gastric cancer can promote the migration and proliferation of gastric cancer cells.
CONCLUSION CONCLUSIONS
Our data suggest that IGF2 is involved in the occurrence and development of gastric cancer. Targeting IGF2 may be a potential therapeutic target for gastric cancer.

Identifiants

DOI: 10.3233/CBM-230105 PMID: 37718779
pubmed: 37718779
pii: CBM230105
doi: 10.3233/CBM-230105
doi:

Substances chimiques

Somatomedins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

355-366

Auteurs

Jiting Sun (J)

Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, China.

Jun Shu (J)

Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, China.
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, and Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China.

Duo Shi (D)

Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, China.

Wen Liu (W)

Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, China.

Yan Zhang (Y)

Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, China.
Department of Clinical Laboratory, Zibo Central Hospital, Zibo, Shandong, China.

Bing Luo (B)

Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, China.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Effects of methylation and imprinting...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Grossesse › Effects of methylation and imprinting...
questionsmedicales.fr › Phénomènes génétiques › Méthylation de l'ADN › Effects of methylation and imprinting...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Empreinte génomique › Effects of methylation and imprinting...
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies gastro-intestinales › Maladies de l'estomac › Tumeurs de l'estomac › Effects of methylation and imprinting...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus oncogènes › Infections à virus Epstein-Barr › Effects of methylation and imprinting...
questionsmedicales.fr › Virus › Virus oncogènes › Virus tumoraux à ADN › Gammaherpesvirinae › Lymphocryptovirus › Herpèsvirus humain de type 4 › Effects of methylation and imprinting...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Somatomédines › Effects of methylation and imprinting...