A 16-year bicentric retrospective analysis of ovarian tissue cryopreservation in pediatric units: indications, results, and outcome.


Journal

Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782

Informations de publication

Date de publication:
2023
Historique:
received: 03 02 2023
accepted: 09 08 2023
medline: 19 9 2023
pubmed: 18 9 2023
entrez: 18 9 2023
Statut: epublish

Résumé

Cancer treatments of the last decades improve the survival rate of children and adolescents. However, chemo- and radiotherapy result in gonadal damage, leading to acute ovarian failure and sterility. The preservation of fertility is now an integral part of care of children requiring gonadotoxic treatments. Ovarian tissue cryopreservation (OTC) is an effective fertility preservation option that allows long-term storage of primordial follicles, subsequent transplantation, and restoration of endocrine function and fertility. The efficacy of this technique is well-demonstrated in adults but the data are scarce for pediatric patients. Currently, OTC represents the only possibility of preserving the potential fertility in prepubertal girls. This is a retrospective study of OTC practice of two French centers from January 2004 to May 2020. A total of 72 patients from pediatric units underwent cryopreservation of ovarian tissue before gonadotoxic therapy for malignant or non-malignant diseases. The ovarian cortex was cut into fragments and the number of follicles per square millimeter was evaluated histologically. The long-term follow-up includes survival rate and hormonal and fertility status. The mean age of patients at OTC was 9.3 years [0.2-17] and 29.2% were postpubertal; 51 had malignant diseases and 21 had non-malignant diseases. The most frequent diagnoses included acute leukemia, hemoglobinopathies, and neuroblastoma. Indication for OTC was stem cell transplantation for 81.9% ( OTC should be proposed to all girls with high risk of developing POI following gonadotoxic therapies in order to give them the possibility of fertility and endocrine restoration.

Sections du résumé

Background
Cancer treatments of the last decades improve the survival rate of children and adolescents. However, chemo- and radiotherapy result in gonadal damage, leading to acute ovarian failure and sterility. The preservation of fertility is now an integral part of care of children requiring gonadotoxic treatments. Ovarian tissue cryopreservation (OTC) is an effective fertility preservation option that allows long-term storage of primordial follicles, subsequent transplantation, and restoration of endocrine function and fertility. The efficacy of this technique is well-demonstrated in adults but the data are scarce for pediatric patients. Currently, OTC represents the only possibility of preserving the potential fertility in prepubertal girls.
Procedure
This is a retrospective study of OTC practice of two French centers from January 2004 to May 2020. A total of 72 patients from pediatric units underwent cryopreservation of ovarian tissue before gonadotoxic therapy for malignant or non-malignant diseases. The ovarian cortex was cut into fragments and the number of follicles per square millimeter was evaluated histologically. The long-term follow-up includes survival rate and hormonal and fertility status.
Results
The mean age of patients at OTC was 9.3 years [0.2-17] and 29.2% were postpubertal; 51 had malignant diseases and 21 had non-malignant diseases. The most frequent diagnoses included acute leukemia, hemoglobinopathies, and neuroblastoma. Indication for OTC was stem cell transplantation for 81.9% (
Conclusion
OTC should be proposed to all girls with high risk of developing POI following gonadotoxic therapies in order to give them the possibility of fertility and endocrine restoration.

Identifiants

pubmed: 37720539
doi: 10.3389/fendo.2023.1158405
pmc: PMC10501795
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1158405

Informations de copyright

Copyright © 2023 Grellet-Grün, Delepine, Le Van Quyen, Avérous, Durlach, Greze, Ladureau-Fritsch, Lichtblau, Canepa, Liné, Paillard, Pluchart, Pirrello, Rongieres, Harika, Becmeur and Teletin.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Marine Grellet-Grün (M)

Department of Reproductive Biology - Centre d'Etude et de Conservation des Oeufs et du Sperme Humain (CECOS), Centre Hospitalier Universitaire de Reims, Reims, France.

Béatrice Delepine (B)

Department of Reproductive Biology - Centre d'Etude et de Conservation des Oeufs et du Sperme Humain (CECOS), Centre Hospitalier Universitaire de Reims, Reims, France.

Pauline Le Van Quyen (P)

Department of Pathology, Hôpital de Hautepierre, Strasbourg, France.

Gerlinde Avérous (G)

Department of Pathology, Hôpital de Hautepierre, Strasbourg, France.

Anne Durlach (A)

Department of Pathology, Centre Hospitalier Universitaire de Reims, Reims, France.

Cécile Greze (C)

Department of Reproductive Biology - Centre d'Etude et de Conservation des Oeufs et du Sperme Humain (CECOS), Centre Médico-chirurgical Obstétrique, Schiltigheim-Strasbourg, France.

Laetitia Ladureau-Fritsch (L)

Department of Reproductive Biology - Centre d'Etude et de Conservation des Oeufs et du Sperme Humain (CECOS), Centre Médico-chirurgical Obstétrique, Schiltigheim-Strasbourg, France.

Isabelle Lichtblau (I)

Department of Reproductive Biology - Centre d'Etude et de Conservation des Oeufs et du Sperme Humain (CECOS), Centre Médico-chirurgical Obstétrique, Schiltigheim-Strasbourg, France.

Anne-Sophie Canepa (AS)

Department of Reproductive Biology - Centre d'Etude et de Conservation des Oeufs et du Sperme Humain (CECOS), Centre Hospitalier Universitaire de Reims, Reims, France.

Antoine Liné (A)

Department of Pediatric Surgery, Centre Hospitalier Universitaire de Reims, Reims, France.

Catherine Paillard (C)

Department of Pediatric Onco-Hematology, Hôpital de Hautepierre, Strasbourg, France.

Claire Pluchart (C)

Department of Pediatric Onco-Hematology, Centre Hospitalier Universitaire de Reims, Reims, France.

Olivier Pirrello (O)

Department of Gynecology-Obstetric, Centre Médico-Chirurgical Obstétrique, Schiltigheim-Strasbourg, France.

Catherine Rongieres (C)

Department of Gynecology-Obstetric, Centre Médico-Chirurgical Obstétrique, Schiltigheim-Strasbourg, France.

Ghassan Harika (G)

Department of Gynecology-Obstetric, Centre Hospitalier Universitaire de Reims, Reims, France.

François Becmeur (F)

Department of Pediatric Surgery, Hôpital de Hautepierre, Strasbourg, France.

Marius Teletin (M)

Department of Reproductive Biology - Centre d'Etude et de Conservation des Oeufs et du Sperme Humain (CECOS), Centre Médico-chirurgical Obstétrique, Schiltigheim-Strasbourg, France.
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS UMR7104), Institut National de la Sante et de la Recherche Médicale (INSERM U1258), Université de Strasbourg (UNISTRA), Illkirch Graffenstaden, France.

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