Mapping protein dynamics at high spatial resolution with temperature-jump X-ray crystallography.
Journal
Nature chemistry
ISSN: 1755-4349
Titre abrégé: Nat Chem
Pays: England
ID NLM: 101499734
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
16
11
2022
accepted:
17
08
2023
medline:
6
11
2023
pubmed:
19
9
2023
entrez:
18
9
2023
Statut:
ppublish
Résumé
Understanding and controlling protein motion at atomic resolution is a hallmark challenge for structural biologists and protein engineers because conformational dynamics are essential for complex functions such as enzyme catalysis and allosteric regulation. Time-resolved crystallography offers a window into protein motions, yet without a universal perturbation to initiate conformational changes the method has been limited in scope. Here we couple a solvent-based temperature jump with time-resolved crystallography to visualize structural motions in lysozyme, a dynamic enzyme. We observed widespread atomic vibrations on the nanosecond timescale, which evolve on the submillisecond timescale into localized structural fluctuations that are coupled to the active site. An orthogonal perturbation to the enzyme, inhibitor binding, altered these dynamics by blocking key motions that allow energy to dissipate from vibrations into functional movements linked to the catalytic cycle. Because temperature jump is a universal method for perturbing molecular motion, the method demonstrated here is broadly applicable for studying protein dynamics.
Identifiants
pubmed: 37723259
doi: 10.1038/s41557-023-01329-4
pii: 10.1038/s41557-023-01329-4
pmc: PMC10624634
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1549-1558Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM117126
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM149436
Pays : United States
Informations de copyright
© 2023. The Author(s).
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