Verification of the efficacy of deciduae for determining the genetic type of mouse embryos.
PCR
decidua
embryo
genotype
mouse
Journal
Anatomia, histologia, embryologia
ISSN: 1439-0264
Titre abrégé: Anat Histol Embryol
Pays: Germany
ID NLM: 7704218
Informations de publication
Date de publication:
19 Sep 2023
19 Sep 2023
Historique:
received:
28
05
2023
accepted:
10
09
2023
medline:
19
9
2023
pubmed:
19
9
2023
entrez:
19
9
2023
Statut:
aheadofprint
Résumé
Mouse embryos in the early-implantation stage require manipulation under a microscope. While the extraction of DNA, RNA and proteins from a single sample allows for both determination of genetic type and analysis of gene expression, whole mount analysis is not possible. In this study, we explored the applicability of PCR using extraembryonic tissues, especially the decidual side tissue after isolating the embryos from implantation sites to establish a method for determining the genetic type of embryos. The implantation site was resected at each day from the date of vaginal plug confirmation, separated into embryos and deciduae. Genomic DNA were isolated separately from the embryos and the deciduae. PCR was performed using these genomic DNA, and the band patterns were compared after electrophoresis. As a result, we demonstrated that detecting embryo-derived cells in the decidua allows determination of the sex and presence of transgenes without harming the mouse embryos themselves, from 8.5 days of age. This method enables the determination of the genetic type of mouse embryos without damaging. This technique would expand the adaptations for analysis of mouse implanted embryos.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
Informations de copyright
© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.
Références
Barratt, K. S., & Arkell, R. M. (2020). Whole-mount in situ hybridization in post-implantation staged mouse embryos. Current Protocol Mouse Biology, 10, e75. https://doi.org/10.1002/cpmo.75
Behringer, R., Gertsenstein, M., Nagy, K. V., & Nagy, A. (2014). Manipulating the mouse embryo: A laboratory manual (Fourth ed.). Cold Spring Harbor Laboratory Press.
Blanco, M. J., Learte, A. I. R., Marchena, M. A., Munoz-Saez, E., Cid, M. A., Rodriguez-Martin, I., & Sanchez-Camacho, C. (2018). Tracing gene expression through detection of detection of β-galactosidase activity in whole mouse embryos. Journal of Visualized Experiments, 2018(136), e57785. https://doi.org/10.3791/57785
Chomczynski, P. (1993). A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques, 15, 536-537.
Hirata, W., Tomoda, T., Yuri, S., & Isotani, A. (2022). Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage. Experimental Animals, 71, 82-89. https://doi.org/10.1538/expanim.21-0119
Hu, D., & Cross, J. C. (2010). Development and function of trophoblast giant cells in the rodent placenta. The International Journal of Developmental Biology, 54, 341-354. https://doi.org/10.1387/ijdb.082768dh
Kaufman, M. H. (1995). The Atlas of Mouse Development (Revised ed.). Academic Press.
Li, Y., Sun, X., & Dey, S. K. (2015). Entosis allows timely elimination of the luminal epithelial barrier for embryo implantation. Cell Reports, 11, 358-365. https://doi.org/10.1016/j.celrep.2015.03.035
Papaioannou, V. E., & Behringer, R. R. (2005). Mouse Phenotypes: A Handbook of Mutation Analysis. Cold Spring Harbor Laboratory Press.
Rossant, J. (1995). Development of the extraembryonic lineages. Seminars in Developmental Biology, 6, 237-247. https://doi.org/10.1016/S1044-5781(06)80049-6
Schlafke, S., & Enders, A. C. (1975). Cellular basis of interaction between trophoblast and uterus at implantation. Biology of Reproduction, 12, 41-65. https://doi.org/10.1095/biolreprod12.1.41
Theiler, K. (1989). The House Mouse: Atlas of Embryonic Development. Springer-Verlag.
Zhao, X., Wei, W., Pan, H., Nie, J., Chen, D., Zhang, P., Chen, F., Fu, Q., Zuo, E., Lu, Y., & Zhang, M. (2019). Identification of the sex of pre-implantation mouse embryos using a marked Y chromosome and CRISPR/Cas9. Scientific Reports, 9, 14315. https://doi.org/10.1038/s41598-019-50731-x