Focusing on the accumulation of chromatin/chromosomes around nucleoli and optimizing the timing of ICSI to facilitate the rescue in vitro maturation of denuded GV stage oocytes.
Accumulation of chromatin/chromosomes
Germinal vesicle
ICSI
Nucleolus
Pregnancy
Time lapse observation
Journal
Journal of assisted reproduction and genetics
ISSN: 1573-7330
Titre abrégé: J Assist Reprod Genet
Pays: Netherlands
ID NLM: 9206495
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
04
05
2023
accepted:
21
08
2023
pmc-release:
01
11
2024
medline:
15
11
2023
pubmed:
19
9
2023
entrez:
19
9
2023
Statut:
ppublish
Résumé
This study aims to achieve the methodological improvement of rescue IVM by predicting germinal vesicle breakdown (GVBD) and optimizing the timing of ICSI. Time lapse analysis was performed retrospectively to evaluated the relationship between the presence of AC around the nucleoli and GVBD. To find the optimal timing of ICSI, the time from the initiation of the first polar body extrusion to ICSI were measured, and the rates of fertilization at each point were calculated. The GVBD rate of GV stage oocytes with AC around the nucleoli was significantly higher than that of GV stage oocytes without AC. The GV stage oocytes required more time for nuclear maturation after polar body extrusion than MI oocytes, with GV stage oocytes taking 400-600 min from polar body extrusion to the optimal timing of ICSI, while the MI stage oocytes took 200-400 min. The GV stage oocytes resulted in the birth of healthy babies with the appropriate timing of ICSI. It was found that GV stage oocytes with AC around nucleoli can initiate GVBD and reach the MII stage with a high rate, and that GV stage oocytes required more time than MI stage oocytes to reach the optimal timing of ICSI. Considering these factors, ART laboratories may employ immature GV stage oocytes in routine ART procedures rather than discarding them.
Identifiants
pubmed: 37725177
doi: 10.1007/s10815-023-02921-w
pii: 10.1007/s10815-023-02921-w
pmc: PMC10643760
doi:
Substances chimiques
Chromatin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2557-2564Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Cha KY, Chian RC. Maturation in vitro of immature human oocytes for clinical use. Hum Reprod Update. 1998;4:103–20. https://doi.org/10.1093/humupd/4.2.103 .
doi: 10.1093/humupd/4.2.103
pubmed: 9683349
De Vos A, Van de Velde H, Joris H, Van Steirteghem A. In-vitro matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection. Hum Reprod. 1999;14:1859–63. https://doi.org/10.1093/humrep/14.7.1859 .
doi: 10.1093/humrep/14.7.1859
pubmed: 10402405
Jee BC, Han SH, Moon JH, Suh CS, Kim SH. Influence of well defined protein source on in vitro maturation of human oocyte: human follicular fluid versus human serum albumin. Fertil Steril. 2008;89:348–52. https://doi.org/10.1016/j.fertnstert.2007.02.052 .
doi: 10.1016/j.fertnstert.2007.02.052
pubmed: 17482173
Huddleston HG, Jackson KV, Doyle JO, Racowsky C. hMG increases the yield of mature oocytes and excellent-quality embryos in patients with a previous cycle having a high incidence of oocyte. Fertil Steril. 2009;92(3):946–9. https://doi.org/10.1016/j.fertnstert.2009.02.039 .
doi: 10.1016/j.fertnstert.2009.02.039
pubmed: 19356754
Edirisinghe WR, Junk SM, Matson PL, Yovich JL. Birth from cryopreserved embryos following in-vitro maturation of oocytes and intracytoplasmic sperm injection. Hum Reprod. 1997;12(5):1056–8. https://doi.org/10.1093/humrep/12.5.1056 .
doi: 10.1093/humrep/12.5.1056
pubmed: 9194665
Son W-Y, Lee S-Y, Lim J-H. Fertilization, cleavage and blastocyst development according to the maturation timing of oocytes in in vitro maturation cycles. Hum Reprod. 2005;20(11):3204–7. https://doi.org/10.1093/humrep/dei195 .
doi: 10.1093/humrep/dei195
pubmed: 16037117
Otsuki J, Momma Y, Takahashi K, Miyakura S, Nagai Y. Timed IVM followed by ICSI in a patient with immature ovarian oocytes. Reprod Biomed Online. 2006;13(1):101–3. https://doi.org/10.1016/s1472-6483(10)62022-6 .
doi: 10.1016/s1472-6483(10)62022-6
pubmed: 16820119
Nagy ZP, Cecile J, Liu J, Loccufier A, Devroey P, Steirteghem AV. Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report. Fertil Steril. 1996;65(5):1047–50. https://doi.org/10.1016/S0015-0282(16)58285-5 .
doi: 10.1016/S0015-0282(16)58285-5
pubmed: 8612833
Escrich L, Galiana Y, Grau N, Insua F, Soler N, Pellicer A, Escribá MJ. Do immature and mature sibling oocytes recovered from stimulated cycles have the same reproductive potential? Reprod Biomed Online. 2018;37(6):667–76. https://doi.org/10.1016/j.rbmo.2018.08.023 .
doi: 10.1016/j.rbmo.2018.08.023
pubmed: 30539737
Vlaisavljević V, Kovac V, Sajko MC. Impact of insulin resistance on the developmental potential of immature oocytes retrieved from human chorionic gonadotropin-primed women with polycystic ovary syndrome undergoing in vitro maturation. Fertil Steril. 2009;91(3):957–9. https://doi.org/10.1016/j.fertnstert.2007.12.062 .
doi: 10.1016/j.fertnstert.2007.12.062
pubmed: 18321495
Tannus S, Hatirnaz S, Tan J, Ata B, Tan SL, Hatirnaz E, Kenat-Pektas M, Dahan MH. Predictive factors for live birth after in vitro maturation of oocytes in women with polycystic ovary syndrome. Arch Gynecol Obstet. 2018;297(1):199–204. https://doi.org/10.1007/s00404-017-4561-z .
doi: 10.1007/s00404-017-4561-z
pubmed: 28993961
Madkour A, Bouamoud N, Kaarouch I, Louanjli N, Saadani B, Assou S, Aboulmaouahib S, Sefrioui O, Amzazi S, Copin H, Benkhalifa M. Follicular fluid and supernatant from cultured cumulus-granulosa cells improve in vitro maturation in patients with polycystic ovarian syndrome. Fertil Steril. 2018;110(4):710–9. https://doi.org/10.1016/j.fertnstert.2018.04.038 .
doi: 10.1016/j.fertnstert.2018.04.038
pubmed: 30196968
Le Du A, Kadoch IJ, Bourcigaux N, Doumerc S, Bourrier M-C, Chevalier N, Fanchin R, Chian R-C, Tachdjian G, Frydman R, Frydman N. In vitro oocyte maturation for the treatment of infertility associated with polycystic ovarian syndrome: the French experience. Hum Reprod. 2005;20(2):420–4. https://doi.org/10.1093/humrep/deh603 .
doi: 10.1093/humrep/deh603
pubmed: 15528263
Sánchez F, Lolicato F, Romero S, De Vos M, Van Ranst H, Verheyen G, Anckaert E, Smitz JEJ. An improved IVM method for cumulus-oocyte complexes from small follicles in polycystic ovary syndrome patients enhances oocyte competence and embryo yield. Hum Reprod. 2017;32(10):2056–68. https://doi.org/10.1093/humrep/dex262 .
doi: 10.1093/humrep/dex262
pubmed: 28938744
Ranganath A, Appaneravanda LC, Gerstl B, Math NT, Menon J, Gunasheela D. A Study to Find Optimal Intra-cytoplasmic Sperm Injection Timing of Oocytes Matured from Germinal Vesicle in in Vitro Maturation Cycles Using a Time Lapse System. Hum Reprod. 2021;14(4):415–21. https://doi.org/10.4103/jhrs.jhrs_130_21 .
doi: 10.4103/jhrs.jhrs_130_21
Kim B-K, Lee S-C, Kim K-J, Han C-H, Kim J-H. In vitro maturation, fertilization, and development of human germinal vesicle oocytes collected from stimulated cycles. Fertil Steril. 2000;74(6):1153–8. https://doi.org/10.1016/s0015-0282(00)01617-4 .
doi: 10.1016/s0015-0282(00)01617-4
pubmed: 11119743
De Vos M, Grynberg M, Ho TM, Yuan Y, Albertini DF, Gilchrist RB. Perspectives on the development and future of oocyte IVM in clinical practice. J Assist Reprod Genet. 2021;38(6):1265–80. https://doi.org/10.1007/s10815-021-02263-5 .
doi: 10.1007/s10815-021-02263-5
pubmed: 34218388
pmcid: 8266966
Jie H, Zhao M, Alqawasmeh OAM, Chan CPS, Lee TL, Li T, Chan DYL. In vitro rescue immature oocytes - a literature review. Fertil Steril. 2021;1–20. https://www.tandfonline.com/doi/full/10.1080/14647273.2021.1876932 . Accessed 16 June 2023
Jones GM, Cram DS, Song B, Magli MC, Gianaroli L, Lacham-Kaplan O, Findlay JK, Jenkin G, Trounson AO. Gene expression profiling of human oocytes following in vivo or in vitro maturation. Hum Reprod. 2008;23:1138–44. https://doi.org/10.1093/humrep/den085 .
doi: 10.1093/humrep/den085
pubmed: 18346995
Otsuki J, Nagai Y. A phase of chromosome aggregation during meiosis in human oocytes. Reprod Biomed Online. 2007;15(2):191–7. https://doi.org/10.1016/s1472-6483(10)60708-0 .
doi: 10.1016/s1472-6483(10)60708-0
pubmed: 17697496
Holubcova Z, Blayney M, Elder K, Schuh M. Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes. Science. 2015;348(6239):1143–7. https://doi.org/10.1126/science.aaa9529 .
doi: 10.1126/science.aaa9529
pubmed: 26045437
pmcid: 4477045
Harasimov K, Uraji J, Mönnich EU, Holubcová Z, Elder K, Blayney M, Schuh M. Actin-driven chromosome clustering facilitates fast and complete chromosome capture in mammalian oocytes. Nat Cell Biol. 2023;25(3):439–52. https://doi.org/10.1038/s41556-022-01082-9 .
doi: 10.1038/s41556-022-01082-9
pubmed: 36732633
pmcid: 10014578
Salimov D, Lopata A, Nagai Y, Lisovskaya T, Portnov I, Miwa A, Otuki J. In human oocytes the aggregation of chromosomes around the nucleolus is associated with the initiation and completion of metiotic maturation. abstract of ASRM. 2014. https://www.fertstert.org/article/S0015-0282(14)01033-4 . Accessed 2 May 2023
Parfenov V, Potchukalina G, Dudina L, Kostyuchek D, Gruzova M. Human antral follicles: oocyte nucleus and the karyosphere formation (electron microscopic and autoradiographic data). Gamete Res. 1989;22(2):219–31. https://doi.org/10.1002/mrd.1120220209 .
doi: 10.1002/mrd.1120220209
pubmed: 2468591
Mattson BA, Albertini DE. Oogenesis: chromatin and microtubule dynamics during meiotic prophase. Mol Reprod Dev. 1990;25(4):374–83. https://doi.org/10.1002/mrd.1080250411 .
doi: 10.1002/mrd.1080250411
pubmed: 1691651
Debey P, Szöllösi MS, Szöllösi D, Vautier D, Girousse A, Besombes D. Competent mouse oocytes isolated from antral follicles exhibit different chromatin organization and follow different maturation dynamics. Mol Reprod Dev. 1993;36(1):59–74. https://doi.org/10.1002/mrd.1080360110 .
doi: 10.1002/mrd.1080360110
pubmed: 8398131
Zuccotti M, Piccinelli A, Giorgi Rossi P, Garagna S, Redi CA. Chromatin organization during mouse oocyte growth. Mol Reprod Dev. 1995;41(4):479–85. https://doi.org/10.1002/mrd.1080410410 .
doi: 10.1002/mrd.1080410410
pubmed: 7576615
Zuccotti M, Giorgi Rossi P, Martinez A, Garagna S, Forabosco A, Redi CA. Meiotic and developmental competence of mouse antral oocytes. Biol Reprod. 1998;58(3):700–4. https://doi.org/10.1095/biolreprod58.3.700 .
doi: 10.1095/biolreprod58.3.700
pubmed: 9510956
Christians E, Boiani M, Garagna S, Dessy C, Redi CA, Renard JP, Zuccotti M. Gene expression and chromatin organization during mouse oocyte growth. Dev Biol. 1999;207(1):76–85. https://doi.org/10.1006/dbio.1998.9157 .
doi: 10.1006/dbio.1998.9157
pubmed: 10049566
Montag M, Schimming T, van der Ven H. Spindle imaging in human oocytes: the impact of the meiotic cell cycle. Reprod Biomed Online. 2006;12(4):442–6. https://doi.org/10.1016/s1472-6483(10)61996-7 .
doi: 10.1016/s1472-6483(10)61996-7
pubmed: 16740216
Yang Q, Zhu L, Wang M, Huang Bo, Li Z, Juan Hu, Xi Q, Liu J, Jin L. Analysis of maturation dynamics and developmental competence of in vitro matured oocytes under time-lapse monitoring. Reprod Biol Endocrinol. 2021;19(1):183. https://doi.org/10.1186/s12958-021-00868-0 .
doi: 10.1186/s12958-021-00868-0
pubmed: 34893069
pmcid: 8662918
Nogueira D, Staessen C, Van de Velde H, Van Steirteghem A. Nuclear status and cytogenetics of embryos derived from in vitro-matured oocytes. Fertil Steril. 2000;74:295–8. https://doi.org/10.1016/s0015-0282(00)00642-7 .
doi: 10.1016/s0015-0282(00)00642-7
pubmed: 10927047
Conti M, Franciosi F. Acquisition of oocyte competence to develop as an embryo: integrated nuclear and cytoplasmic events. Hum Reprod Update. 2018;24(3):245–66. https://doi.org/10.1093/humupd/dmx040 .
doi: 10.1093/humupd/dmx040
pubmed: 29432538
pmcid: 5907346