Compact portable higher harmonic generation microscopy for the real time assessment of unprocessed thyroid tissue.
autofluorescence
instant pathology
label-free
portable microscope
second harmonic generation
third harmonic generation
thyroid
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
19 Sep 2023
19 Sep 2023
Historique:
revised:
12
09
2023
received:
09
03
2023
accepted:
15
09
2023
pubmed:
19
9
2023
medline:
19
9
2023
entrez:
19
9
2023
Statut:
aheadofprint
Résumé
During thyroid surgery fast and reliable intra-operative pathological feedback has the potential to avoid a two-stage procedure and significantly reduce health care costs in patients undergoing a diagnostic hemithyroidectomy (HT). We explored higher harmonic generation (HHG) microscopy, which combines second harmonic generation (SHG), third harmonic generation (THG), and multiphoton excited autofluorescence (MPEF) for this purpose. With a compact, portable HHG microscope, images of freshly excised healthy tissue, benign nodules (follicular adenoma) and malignant tissue (papillary carcinoma, follicular carcinoma and spindle cell carcinoma) were recorded. The images were generated on unprocessed tissue within minutes and show relevant morphological thyroid structures in good accordance with the histology images. The thyroid follicle architecture, cells, cell nuclei (THG), collagen organization (SHG) and the distribution of thyroglobulin and/or thyroid hormones T3 or T4 (MPEF) could be visualized. We conclude that SHG/THG/MPEF imaging is a promising tool for clinical intraoperative assessment of thyroid tissue.
Identifiants
pubmed: 37725434
doi: 10.1002/jbio.202300079
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300079Subventions
Organisme : Dutch Research Council (NWO)
ID : 15825
Informations de copyright
© 2023 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
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