RNA-based medicine: from molecular mechanisms to therapy.

CRISPR-Cas therapy RNA interference antisense oligonucleotide drug mRNA therapeutics rare genetic disease

Journal

The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664

Informations de publication

Date de publication:
02 11 2023
Historique:
revised: 24 08 2023
received: 14 06 2023
accepted: 29 08 2023
medline: 3 11 2023
pubmed: 20 9 2023
entrez: 20 9 2023
Statut: ppublish

Résumé

RNA-based therapeutics have the potential to revolutionize the treatment and prevention of human diseases. While early research faced setbacks, it established the basis for breakthroughs in RNA-based drug design that culminated in the extraordinarily fast development of mRNA vaccines to combat the COVID-19 pandemic. We have now reached a pivotal moment where RNA medicines are poised to make a broad impact in the clinic. In this review, we present an overview of different RNA-based strategies to generate novel therapeutics, including antisense and RNAi-based mechanisms, mRNA-based approaches, and CRISPR-Cas-mediated genome editing. Using three rare genetic diseases as examples, we highlight the opportunities, but also the challenges to wide-ranging applications of this class of drugs.

Identifiants

pubmed: 37728251
doi: 10.15252/embj.2023114760
pmc: PMC10620767
doi:

Substances chimiques

RNA 63231-63-0

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e114760

Informations de copyright

© 2023 The Authors. Published under the terms of the CC BY 4.0 license.

Références

Science. 1999 Oct 29;286(5441):950-2
pubmed: 10542148
Mol Pharmacol. 1995 Nov;48(5):905-18
pubmed: 7476922
Proc Natl Acad Sci U S A. 2022 Sep 27;119(39):e2210104119
pubmed: 36122230
Am J Ophthalmol. 2002 Apr;133(4):467-74
pubmed: 11931780
Science. 2012 Apr 13;336(6078):237-40
pubmed: 22499947
Immunity. 2005 Aug;23(2):165-75
pubmed: 16111635
Nat Med. 2019 Feb;25(2):229-233
pubmed: 30664785
Science. 2018 Mar 23;359(6382):1355-1360
pubmed: 29567706
Nat Methods. 2013 Oct;10(10):977-9
pubmed: 23892898
Nat Methods. 2013 Oct;10(10):973-6
pubmed: 23892895
Hum Gene Ther. 2002 Jul 20;13(11):1349-59
pubmed: 12162817
Science. 1989 Sep 8;245(4922):1066-73
pubmed: 2475911
Nat Struct Mol Biol. 2015 Jan;22(1):20-8
pubmed: 25565029
Nucleic Acids Res. 2008 Oct;36(18):5812-21
pubmed: 18782830
Nature. 2000 Mar 16;404(6775):293-6
pubmed: 10749213
Lancet. 2019 Nov 30;394(10213):2025-2038
pubmed: 31789220
Nucleic Acids Res. 2013 Jul;41(12):6209-21
pubmed: 23620279
Mol Pharmacol. 1994 May;45(5):932-43
pubmed: 8190109
Nat Biotechnol. 2017 Mar;35(3):238-248
pubmed: 28244990
Nat Rev Drug Discov. 2023 Aug;22(8):607-609
pubmed: 37438497
Nat Biotechnol. 2022 Nov;40(11):1586-1600
pubmed: 36329321
Nucleic Acids Res. 2023 Apr 11;51(6):2529-2573
pubmed: 36881759
Mol Ther Nucleic Acids. 2022 Jun 22;29:135-149
pubmed: 35847173
Chem Biol. 2001 Jan;8(1):1-7
pubmed: 11182314
Curr Opin Microbiol. 2016 Oct;33:47-55
pubmed: 27375107
Chembiochem. 2015 Apr 13;16(6):903-8
pubmed: 25786782
J Biol Chem. 1997 May 2;272(18):11994-2000
pubmed: 9115264
Nucleic Acids Res. 2015 Jan;43(2):1189-203
pubmed: 25550431
Nat Genet. 2023 Jul;55(7):1210-1220
pubmed: 37400614
J Pharmacol Exp Ther. 2000 Feb;292(2):468-79
pubmed: 10640282
JAMA Neurol. 2020 Sep 1;77(9):1122-1131
pubmed: 32539076
Nat Genet. 2002 Apr;30(4):377-84
pubmed: 11925564
Cell. 2010 Mar 19;140(6):805-20
pubmed: 20303872
Mol Microbiol. 2020 Mar;113(3):550-559
pubmed: 32185839
Proc Natl Acad Sci U S A. 1978 Jan;75(1):280-4
pubmed: 75545
Proc Natl Acad Sci U S A. 1985 Jan;82(1):144-8
pubmed: 3855537
Proc Natl Acad Sci U S A. 2012 Sep 25;109(39):E2579-86
pubmed: 22949671
Amyloid. 2023 Mar;30(1):1-9
pubmed: 35875890
Cell. 2021 Oct 28;184(22):5635-5652.e29
pubmed: 34653350
Nat Rev Drug Discov. 2017 Mar;16(3):203-222
pubmed: 28209991
Science. 2016 Jan 22;351(6271):400-3
pubmed: 26721683
Nucleic Acids Res. 2017 Sep 19;45(16):9528-9546
pubmed: 28934489
N Engl J Med. 2021 Jan 21;384(3):252-260
pubmed: 33283989
EMBO J. 2023 Nov 2;42(21):e114760
pubmed: 37728251
Nat Rev Mater. 2021;6(12):1078-1094
pubmed: 34394960
Nature. 2020 Feb;578(7794):229-236
pubmed: 32051598
Nature. 2016 Jun 01;534(7607):396-401
pubmed: 27281205
Muscle Nerve. 2021 Sep;64(3):285-292
pubmed: 34105177
Front Immunol. 2021 Jan 28;11:625833
pubmed: 33633744
Nat Rev Immunol. 2022 Apr;22(4):224-235
pubmed: 34272507
Nat Nanotechnol. 2021 Jun;16(6):630-643
pubmed: 34059811
Lancet Diabetes Endocrinol. 2023 Feb;11(2):109-119
pubmed: 36620965
Nature. 2001 May 24;411(6836):494-8
pubmed: 11373684
Cell. 1995 Jan 13;80(1):155-65
pubmed: 7813012
J Hum Genet. 2017 Apr;62(4):459-463
pubmed: 27974813
Lancet. 2017 Dec 17;388(10063):3017-3026
pubmed: 27939059
Medicine (Baltimore). 2019 Jun;98(26):e15858
pubmed: 31261494
Compr Physiol. 2015 Jul 1;5(3):1223-39
pubmed: 26140716
Nat Rev Dis Primers. 2021 Feb 18;7(1):13
pubmed: 33602943
Nature. 2017 Jul 13;547(7662):222-226
pubmed: 28678784
Nat Rev Drug Discov. 2021 Jun;20(6):427-453
pubmed: 33762737
Nature. 2000 Nov 2;408(6808):86-9
pubmed: 11081512
Science. 2005 Sep 2;309(5740):1577-81
pubmed: 16141076
Nucleic Acids Res. 2017 Apr 7;45(6):3528-3536
pubmed: 27903888
Cell Stem Cell. 2021 Jun 3;28(6):1136-1147.e5
pubmed: 33626327
Science. 2020 Apr 17;368(6488):290-296
pubmed: 32217751
Drugs Today (Barc). 2019 Oct;55(10):627-639
pubmed: 31720560
Front Immunol. 2021 Mar 17;12:658399
pubmed: 33815421
RSC Adv. 2021 Jan 11;11(4):2415-2426
pubmed: 35424193
Nat Biotechnol. 2020 Jul;38(7):824-844
pubmed: 32572269
Nat Biotechnol. 2018 Sep;36(8):765-771
pubmed: 30010673
J Biol Chem. 2021 Jan-Jun;296:100416
pubmed: 33600796
Nature. 1983 Feb 3;301(5899):421-2
pubmed: 6823316
ACS Infect Dis. 2018 May 11;4(5):806-814
pubmed: 29461800
Science. 2018 Sep 21;361(6408):1259-1262
pubmed: 30166441
Nucleic Acids Res. 1999 Mar 15;27(6):1547-51
pubmed: 10037819
Cell. 1993 Dec 3;75(5):843-54
pubmed: 8252621
N Engl J Med. 2017 Nov 2;377(18):1723-1732
pubmed: 29091570
Science. 2014 Sep 5;345(6201):1184-1188
pubmed: 25123483
Cell. 2022 Jan 20;185(2):250-265.e16
pubmed: 35021064
Nat Biotechnol. 2020 Apr;38(4):471-481
pubmed: 32042170
Science. 2007 Mar 23;315(5819):1709-12
pubmed: 17379808
Cell. 1993 Dec 3;75(5):855-62
pubmed: 8252622
Nucleic Acids Res. 2001 Mar 15;29(6):1293-9
pubmed: 11238995
Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17337-42
pubmed: 17085592
Proc Natl Acad Sci U S A. 1978 Jan;75(1):285-8
pubmed: 75546
Nat Rev Genet. 2015 Jan;16(1):45-56
pubmed: 25404111
Biochim Biophys Acta. 1999 Dec 10;1489(1):141-58
pubmed: 10807004
Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9618-23
pubmed: 10931943
Cell. 1987 Dec 24;51(6):919-28
pubmed: 3319190
J Cell Biol. 2012 Oct 1;199(1):21-5
pubmed: 23027901
Mol Ther. 2020 Sep 2;28(9):2044-2055
pubmed: 32892813
Nat Rev Genet. 2023 Mar;24(3):161-177
pubmed: 36344749
Nature. 2015 Nov 12;527(7577):192-7
pubmed: 26375006
Am J Hum Genet. 2008 Apr;82(4):834-48
pubmed: 18371932
Nucleic Acids Res. 2011 Nov;39(21):e142
pubmed: 21890902
Nature. 2018 Apr 5;556(7699):57-63
pubmed: 29512652
Science. 2023 Jan 20;379(6629):eadd8643
pubmed: 36656942
J Am Chem Soc. 2014 Dec 10;136(49):16958-61
pubmed: 25434769
Chembiochem. 2016 Jun 2;17(11):985-9
pubmed: 27121751
J Control Release. 2016 Oct 28;240:227-234
pubmed: 26718856
Nature. 2019 Dec;576(7785):149-157
pubmed: 31634902
Nat Rev Drug Discov. 2022 Apr;21(4):261-282
pubmed: 35105974
Sci Adv. 2019 Mar 06;5(3):eaav4324
pubmed: 30854433
Science. 1989 Sep 8;245(4922):1059-65
pubmed: 2772657
N Engl J Med. 2021 Feb 4;384(5):403-416
pubmed: 33378609
Ann Neurol. 2016 Feb;79(2):257-71
pubmed: 26573217
Mol Ther. 2017 Jul 5;25(7):1467-1475
pubmed: 28412170
BMC Genomics. 2018 Mar 21;19(1):212
pubmed: 29562890
J Pharmacol Exp Ther. 2014 Jul;350(1):46-55
pubmed: 24784568
Am J Ophthalmol. 2002 Apr;133(4):475-83
pubmed: 11931781
Cell. 2000 Mar 31;101(1):25-33
pubmed: 10778853
Sci Transl Med. 2011 Mar 2;3(72):72ra18
pubmed: 21368223
Nature. 1998 Feb 19;391(6669):806-11
pubmed: 9486653
Cell. 1993 Jul 2;73(7):1251-4
pubmed: 7686820
N Engl J Med. 2021 Aug 5;385(6):493-502
pubmed: 34215024
Nat Biotechnol. 2016 Aug;34(8):875-80
pubmed: 27398791
Nucleic Acids Res. 1997 Nov 15;25(22):4429-43
pubmed: 9358149
Nature. 2011 Oct 05;478(7367):123-6
pubmed: 21979052
Nat Rev Drug Discov. 2019 Jun;18(6):421-446
pubmed: 30846871
Science. 1983 Sep 9;221(4615):1067-70
pubmed: 6308769
Nat Biotechnol. 2022 Mar;40(3):402-410
pubmed: 34608327
Lancet. 2021 Jun 5;397(10290):2195-2211
pubmed: 34090606
Toxicol Res (Camb). 2018 Mar 22;7(3):473-479
pubmed: 30090597
Nat Rev Genet. 2013 Jul;14(7):447-59
pubmed: 23732335
Cell. 2013 Jul 18;154(2):442-51
pubmed: 23849981
Nat Rev Drug Discov. 2021 Nov;20(11):817-838
pubmed: 34433919
Nucleic Acids Res. 2017 Jul 27;45(13):7581-7592
pubmed: 28591791
Cell. 1991 Feb 22;64(4):681-91
pubmed: 1705179
Science. 2008 Aug 15;321(5891):960-4
pubmed: 18703739
J Cyst Fibros. 2020 Jan;19(1):99-107
pubmed: 31182369
Nature. 2016 Apr 20;533(7603):420-4
pubmed: 27096365
Science. 2010 Jan 8;327(5962):198-201
pubmed: 19965718
Proc Natl Acad Sci U S A. 2021 Jun 1;118(22):
pubmed: 34074727
Science. 2016 Sep 16;353(6305):
pubmed: 27492474
Nat Rev Genet. 2018 Dec;19(12):770-788
pubmed: 30323312
Cell. 2022 Jul 21;185(15):2806-2827
pubmed: 35798006
J Mol Evol. 2005 Feb;60(2):174-82
pubmed: 15791728
Mol Ther Nucleic Acids. 2022 Jan 03;27:797-809
pubmed: 35116191
Nat Biotechnol. 2022 Oct;40(10):1500-1508
pubmed: 35654979
Nat Cell Biol. 2016 Dec 23;19(1):1-9
pubmed: 28008184
Nat Biotechnol. 2019 Aug;37(8):884-894
pubmed: 31375812
Nat Med. 2022 Aug;28(8):1619-1629
pubmed: 35970920
Annu Rev Immunol. 2019 Apr 26;37:349-375
pubmed: 30673536
Nat Rev Drug Discov. 2020 Oct;19(10):673-694
pubmed: 32782413
Mol Cells. 2023 Jan 31;46(1):10-20
pubmed: 36697233
N Engl J Med. 2018 Jul 5;379(1):11-21
pubmed: 29972753
Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9742-7
pubmed: 11481446
Cell Stem Cell. 2020 Apr 2;26(4):503-510.e7
pubmed: 32084388
J Clin Med. 2021 Feb 18;10(4):
pubmed: 33670689
Nucleic Acids Res. 2019 Jul 26;47(13):6900-6916
pubmed: 31165876
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Molecules. 2021 Sep 06;26(17):
pubmed: 34500849
Nature. 2010 Nov 4;468(7320):67-71
pubmed: 21048762
Nat Struct Biol. 2003 Feb;10(2):120-5
pubmed: 12524529
Science. 2004 Sep 3;305(5689):1437-41
pubmed: 15284456
Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4034-9
pubmed: 16495412
Mol Ther. 2021 Nov 3;29(11):3107-3124
pubmed: 34509669
Cell. 2014 Oct 23;159(3):647-61
pubmed: 25307932
Wiley Interdiscip Rev RNA. 2021 Jul;12(4):e1641
pubmed: 33567469
RNA. 2023 Apr;29(4):415-422
pubmed: 36657971
Nat Med. 2019 May;25(5):776-783
pubmed: 30911135
Science. 2013 Feb 15;339(6121):823-6
pubmed: 23287722
Nature. 2011 Mar 31;471(7340):602-7
pubmed: 21455174
N Engl J Med. 2013 May 2;368(18):1685-94
pubmed: 23534542
Science. 2021 Jan 8;371(6525):145-153
pubmed: 33414215
RNA. 2023 Apr;29(4):396-401
pubmed: 36669888
Nucleic Acids Res. 2014 May;42(9):5871-9
pubmed: 24589581
Cell. 1993 Oct 22;75(2):207-16
pubmed: 7691415
ACS Chem Biol. 2015 May 15;10(5):1181-7
pubmed: 25730476
Nucleic Acids Res. 2023 Jul 7;51(12):5901-5910
pubmed: 37224533
Nature. 1990 Sep 27;347(6291):358-63
pubmed: 1699126
Gene Ther. 2021 Sep;28(9):549-559
pubmed: 33574580
Neurology. 2020 May 26;94(21):e2270-e2282
pubmed: 32139505
Nat Chem Biol. 2007 Mar;3(3):166-73
pubmed: 17259978
Nucleic Acids Res. 2015 Mar 31;43(6):2993-3011
pubmed: 25753666
Nature. 2023 Jun;618(7963):144-150
pubmed: 37165196
J Cyst Fibros. 2019 Jul;18(4):536-542
pubmed: 30467074
Nat Med. 2019 Feb;25(2):249-254
pubmed: 30692695
Nat Rev Drug Discov. 2017 Mar;16(3):181-202
pubmed: 27807347
Science. 2013 Feb 15;339(6121):819-23
pubmed: 23287718
Nat Rev Drug Discov. 2022 Aug;21(8):545-547
pubmed: 35831515
N Engl J Med. 2020 Dec 31;383(27):2603-2615
pubmed: 33301246
Cell. 1987 Jul 31;50(3):509-17
pubmed: 3607877
Elife. 2013 Jan 29;2:e00471
pubmed: 23386978
RNA. 2023 Apr;29(4):434-445
pubmed: 36653113
Nucleic Acids Res. 2019 Feb 20;47(3):1082-1096
pubmed: 30544191
Nat Biotechnol. 2001 Apr;19(4):360-4
pubmed: 11283595
Science. 1989 Sep 8;245(4922):1073-80
pubmed: 2570460
Nat Chem Biol. 2012 Apr 15;8(6):555-61
pubmed: 22504300
Science. 2005 Sep 2;309(5740):1573-6
pubmed: 16081698
Mol Ther Methods Clin Dev. 2020 Jun 01;18:464-472
pubmed: 32728595
Science. 2023 Apr 21;380(6642):eadg6518
pubmed: 36996170
Nucleic Acids Res. 2011 Jun;39(11):4795-807
pubmed: 21345934
Nature. 2006 May 25;441(7092):537-41
pubmed: 16724069
Nat Commun. 2019 Aug 7;10(1):3556
pubmed: 31391465
Science. 2002 Sep 20;297(5589):2056-60
pubmed: 12154197

Auteurs

Anke Sparmann (A)

Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany.

Jörg Vogel (J)

Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany.
Institute of Molecular Infection Biology (IMIB), University of Würzburg, Würzburg, Germany.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH