Imaging of fibrogenesis in the liver by [

Fibrogenesis Hepatic stellate cells Liver fibrosis PET imaging Platelet derived growth factor receptor

Journal

EJNMMI radiopharmacy and chemistry
ISSN: 2365-421X
Titre abrégé: EJNMMI Radiopharm Chem
Pays: England
ID NLM: 101714628

Informations de publication

Date de publication:
21 Sep 2023
Historique:
received: 27 07 2023
accepted: 04 09 2023
medline: 21 9 2023
pubmed: 21 9 2023
entrez: 21 9 2023
Statut: epublish

Résumé

Platelet-derived growth factor receptor beta (PDGFRβ) is a receptor overexpressed on activated hepatic stellate cells (aHSCs). Positron emission tomography (PET) imaging of PDGFRβ could potentially allow the quantification of fibrogenesis in fibrotic livers. This study aims to evaluate a fluorine-18 radiolabeled Affibody molecule ([ In vitro specificity studies demonstrated that the trans-Cyclooctenes (TCO) conjugated Z09591 Affibody molecule had a picomolar affinity for human PDGFRβ. Biodistribution performed on healthy rats showed rapid clearance of [ Our study highlights [18F]TZ-Z09591 as a specific tracer for fibrogenic cells in the fibrotic liver, thus offering the potential to assess fibrogenesis clearly.

Sections du résumé

BACKGROUND BACKGROUND
Platelet-derived growth factor receptor beta (PDGFRβ) is a receptor overexpressed on activated hepatic stellate cells (aHSCs). Positron emission tomography (PET) imaging of PDGFRβ could potentially allow the quantification of fibrogenesis in fibrotic livers. This study aims to evaluate a fluorine-18 radiolabeled Affibody molecule ([
RESULTS RESULTS
In vitro specificity studies demonstrated that the trans-Cyclooctenes (TCO) conjugated Z09591 Affibody molecule had a picomolar affinity for human PDGFRβ. Biodistribution performed on healthy rats showed rapid clearance of [
CONCLUSIONS CONCLUSIONS
Our study highlights [18F]TZ-Z09591 as a specific tracer for fibrogenic cells in the fibrotic liver, thus offering the potential to assess fibrogenesis clearly.

Identifiants

pubmed: 37733133
doi: 10.1186/s41181-023-00210-6
pii: 10.1186/s41181-023-00210-6
pmc: PMC10513984
doi:

Types de publication

Journal Article

Langues

eng

Pagination

23

Informations de copyright

© 2023. Springer Nature Switzerland AG.

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Auteurs

Olivia Wegrzyniak (O)

Science for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Dag Hammarskjölds Väg 14C, 3tr, 751 83, Uppsala, Sweden.

Bo Zhang (B)

Science for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Dag Hammarskjölds Väg 14C, 3tr, 751 83, Uppsala, Sweden.

Johanna Rokka (J)

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

Maria Rosestedt (M)

Science for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Dag Hammarskjölds Väg 14C, 3tr, 751 83, Uppsala, Sweden.

Bogdan Mitran (B)

Science for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Dag Hammarskjölds Väg 14C, 3tr, 751 83, Uppsala, Sweden.
Antaros Medical AB, Uppsala, Sweden.

Pierre Cheung (P)

Science for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Dag Hammarskjölds Väg 14C, 3tr, 751 83, Uppsala, Sweden.

Emmi Puuvuori (E)

Science for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Dag Hammarskjölds Väg 14C, 3tr, 751 83, Uppsala, Sweden.

Sofie Ingvast (S)

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

Jonas Persson (J)

Science for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Dag Hammarskjölds Väg 14C, 3tr, 751 83, Uppsala, Sweden.
Department of Protein Science, Division of Protein Engineering, KTH Royal Institute of Technology, Stockholm, Sweden.

Helena Nordström (H)

Science for Life Laboratory, Drug Discovery and Development Platform, Department of Chemistry-BMC, Uppsala University, Uppsala, Sweden.

John Löfblom (J)

Department of Protein Science, Division of Protein Engineering, KTH Royal Institute of Technology, Stockholm, Sweden.

Fredrik Pontén (F)

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

Fredrik Y Frejd (FY)

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.
Affibody AB, Solna, Sweden.

Olle Korsgren (O)

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

Jonas Eriksson (J)

Science for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Dag Hammarskjölds Väg 14C, 3tr, 751 83, Uppsala, Sweden. jonas.p.eriksson@akademiska.se.
Uppsala University Hospital PET Center, Entrance 85, Dag Hammarskjölds Väg 21, 752 37, Uppsala, Sweden. jonas.p.eriksson@akademiska.se.

Olof Eriksson (O)

Science for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Dag Hammarskjölds Väg 14C, 3tr, 751 83, Uppsala, Sweden. olof.eriksson@ilk.uu.se.
Antaros Medical AB, Uppsala, Sweden. olof.eriksson@ilk.uu.se.

Classifications MeSH