Mesenchymal Stem Cells Isolated from Equine Hair Follicles Using a Method of Air-Liquid Interface.
Autologous veterinary therapy
Equine hair follicles
Mesenchymal stem cells
Minimal-invasive cell source
Tri-lineage differentiations
Journal
Stem cell reviews and reports
ISSN: 2629-3277
Titre abrégé: Stem Cell Rev Rep
Pays: United States
ID NLM: 101752767
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
accepted:
28
08
2023
medline:
21
11
2023
pubmed:
21
9
2023
entrez:
21
9
2023
Statut:
ppublish
Résumé
Equine mesenchymal stem cells (MSC) of various origins have been identified in horses, including MSCs from the bone marrow and adipose tissue. However, these stem cell sources are highly invasive in sampling, which thereby limits their clinical application in equine veterinary medicine. This study presents a novel method using an air-liquid interface to isolate stem cells from the hair follicle outer root sheath of the equine forehead skin. These stem cells cultured herewith showed high proliferation and asumed MSC phenotype by expressing MSC positive biomarkers (CD29, CD44 CD90) while not expressing negative markers (CD14, CD34 and CD45). They were capable of differentiating towards chondrogenic, osteogenic and adipogenic lineages, which was comparable with MSCs from adipose tissue. Due to their proliferative phenotype in vitro, MSC-like profile and differentiation capacities, we named them equine mesenchymal stem cells from the hair follicle outer root sheath (eMSCORS). eMSCORS present a promising alternative stem cell source for the equine veterinary medicine.
Identifiants
pubmed: 37733199
doi: 10.1007/s12015-023-10619-w
pii: 10.1007/s12015-023-10619-w
pmc: PMC10661790
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2943-2956Subventions
Organisme : Sächsisches Staatsministerium für Wissenschaft und Kunst
ID : SIKT Teilprojekt 34707001 / SMWK 34707004
Organisme : Natural Science Foundation of Hubei Province
ID : grant No.2021CFB289
Informations de copyright
© 2023. The Author(s).
Références
Equine Vet J. 2013 Jul;45(4):435-41
pubmed: 23173847
Vet Surg. 2014 Mar;43(3):255-65
pubmed: 24433318
Vet Res Commun. 2011 Aug;35(6):355-65
pubmed: 21614641
Stem Cell Rev Rep. 2022 Aug;18(6):2028-2044
pubmed: 35080748
Can Vet J. 2009 Feb;50(2):155-65
pubmed: 19412395
PLoS One. 2016 Jun 03;11(6):e0156821
pubmed: 27257959
Nature. 2007 Jul 19;448(7151):313-7
pubmed: 17554338
Cell Transplant. 2016 Nov;25(11):2003-2016
pubmed: 27226077
BMC Vet Res. 2018 Jan 17;14(1):17
pubmed: 29343270
J Bone Joint Surg Br. 2007 May;89(5):672-85
pubmed: 17540757
Vet Immunol Immunopathol. 2009 Dec 15;132(2-4):303-6
pubmed: 19647331
Regen Med. 2012 Sep;7(5):713-20
pubmed: 22830621
J Bone Joint Surg Am. 2018 Jan 17;100(2):138-146
pubmed: 29342064
Stem Cell Res Ther. 2015 Apr 12;6:54
pubmed: 25889095
BMC Biotechnol. 2007 May 30;7:26
pubmed: 17537254
Cell Transplant. 2019 Jul;28(7):801-812
pubmed: 31018669
J Orthop Res. 2009 Dec;27(12):1675-80
pubmed: 19544397
Cytotherapy. 2011 Apr;13(4):419-30
pubmed: 21105841
Am J Vet Res. 2008 Jul;69(7):928-37
pubmed: 18593247
Front Vet Sci. 2018 Apr 16;5:70
pubmed: 29713634
Arthroscopy. 2011 Nov;27(11):1552-61
pubmed: 21862278
Cytotherapy. 2013 Jul;15(7):753-9
pubmed: 23602595
Reproduction. 2012 Apr;143(4):455-68
pubmed: 22274885
J Orthop Res. 2008 Mar;26(3):322-31
pubmed: 17960654
Regen Med. 2015;10(3):305-15
pubmed: 25933239
PLoS One. 2011;6(5):e19964
pubmed: 21625520
Equine Vet J. 2013 May;45(3):372-5
pubmed: 23009322
Stem Cell Res Ther. 2011 Feb 23;2(1):9
pubmed: 21371354
Vet Q. 2013 Dec;33(4):234-41
pubmed: 23697553
Pol J Vet Sci. 2013;16(4):753-4
pubmed: 24597313
Res Vet Sci. 2013 Aug;95(1):272-7
pubmed: 23419936
J Orthop Res. 2007 Jul;25(7):913-25
pubmed: 17405160
Vet J. 2018 Aug;238:49-57
pubmed: 30103915
Stem Cell Res Ther. 2011 Jan 27;2(1):4
pubmed: 21272343
Cytotechnology. 2012 Jan;64(1):1-7
pubmed: 21994048
Transfus Med Hemother. 2022 May 16;49(3):158-162
pubmed: 35813600
Front Vet Sci. 2015 Dec 07;2:67
pubmed: 26664993
Br J Sports Med. 2005 Sep;39(9):582-4
pubmed: 16118291
Cytotherapy. 2006;8(4):315-7
pubmed: 16923606
Equine Vet J. 1983 Oct;15(4):371-2
pubmed: 6641685
Histol Histopathol. 2010 Oct;25(10):1277-85
pubmed: 20712012
J Transl Med. 2011 Mar 22;9:29
pubmed: 21418664
Front Immunol. 2021 Feb 05;11:591065
pubmed: 33613514
Cells. 2020 Sep 10;9(9):
pubmed: 32927740
Am J Vet Res. 2007 Oct;68(10):1095-105
pubmed: 17916017
Equine Vet J. 2013 Mar;45(2):193-8
pubmed: 22780195
Stem Cell Res Ther. 2018 Jul 4;9(1):178
pubmed: 29973295
Bone Joint Res. 2012 Nov 01;1(11):297-309
pubmed: 23610661
Vet Surg. 2007 Oct;36(7):613-22
pubmed: 17894587
J Bone Joint Surg Am. 2010 Aug 18;92(10):1927-37
pubmed: 20720135
Arthritis Rheum. 2003 Dec;48(12):3464-74
pubmed: 14673997
Stem Cells Transl Med. 2020 Jan;9(1):39-46
pubmed: 31411820
Cytotherapy. 2009;11(4):443-7
pubmed: 19513899
Cytotherapy. 2011 Nov;13(10):1180-92
pubmed: 21899391
Int J Cardiol. 2007 May 2;117(3):360-4
pubmed: 16889857
Front Vet Sci. 2015 Nov 19;2:59
pubmed: 26664986
Vet Surg. 2008 Dec;37(8):713-24
pubmed: 19121166
Plast Reconstr Surg. 2013 Jan;131(1):77e-85e
pubmed: 23271558
Equine Vet J. 2017 Jul;49(4):539-544
pubmed: 27862236
Curr Med Chem. 2010;17(8):759-66
pubmed: 20088756
Vet Clin North Am Equine Pract. 2011 Aug;27(2):263-74
pubmed: 21872758
Biol Blood Marrow Transplant. 2015 Sep;21(9):1545-54
pubmed: 26079441
Cell Cycle. 2019 Nov;18(21):2954-2971
pubmed: 31505996
Am J Vet Res. 2016 Jul;77(7):779-88
pubmed: 27347833