Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device.
Journal
APL bioengineering
ISSN: 2473-2877
Titre abrégé: APL Bioeng
Pays: United States
ID NLM: 101726398
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
01
02
2023
accepted:
08
08
2023
medline:
22
9
2023
pubmed:
22
9
2023
entrez:
22
9
2023
Statut:
epublish
Résumé
We developed an innovative millifluidic organ-on-a-chip device, named MINERVA 2.0, that is optically accessible and suitable to serial connection. In the present work, we evaluated MINERVA 2.0 as millifluidic gut epithelium-on-a-chip by using computational modeling and biological assessment. We also tested MINERVA 2.0 in a serially connected configuration prodromal to address the complexity of multiorgan interaction. Once cultured under perfusion in our device, human gut immortalized Caco-2 epithelial cells were able to survive at least up to 7 days and form a three-dimensional layer with detectable tight junctions (occludin and zonulin-1 positive). Functional layer development was supported by measurable trans-epithelial resistance and FITC-dextran permeability regulation, together with mucin-2 expression. The dynamic culturing led to a specific transcriptomic profile, assessed by RNASeq, with a total of 524 dysregulated transcripts (191 upregulated and 333 downregulated) between static and dynamic condition. Overall, the collected results suggest that our gut-on-a-chip millifluidic model displays key gut epithelium features and, thanks to its modular design, may be the basis to build a customizable multiorgan-on-a-chip platform.
Identifiants
pubmed: 37736017
doi: 10.1063/5.0144862
pii: 5.0144862
pmc: PMC10511260
doi:
Types de publication
Journal Article
Langues
eng
Pagination
036117Informations de copyright
© 2023 Author(s).
Déclaration de conflit d'intérêts
The authors have no conflicts to disclose.
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