Comprehensive analysis on the regulation of differentially expressed of mRNA and ncRNA in different ovarian stages of ark shell Scapharca broughtonii.
CeRNA
Non-coding RNA
Ovarian development
Scapharca broughtonii
Sex steroid hormones
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
22 Sep 2023
22 Sep 2023
Historique:
received:
23
05
2023
accepted:
04
09
2023
medline:
25
9
2023
pubmed:
22
9
2023
entrez:
22
9
2023
Statut:
epublish
Résumé
Ovarian development is an important prerequisite and basis for animal reproduction. In many vertebrates, it is regulated by multiple genes and influenced by sex steroid hormones and environmental factors. However, relative information is limited in shellfish. To explore the biological functions and molecular mechanisms of mRNA and non-coding RNA that regulate ovarian development in Scapharca broughtonii, we performed whole transcriptome sequencing analysis on ovaries at three developmental stages. Furthermore, the biological processes involved in the differential expression of mRNA and ncRNA were analyzed. A total of 11,342 mRNAs, 6897 lncRNAs, 135 circRNAs, and 275 miRNAs were differentially expressed. By mapping the differentially expressed RNAs from the three developmental stages of Venn diagram, multiple groups of shared mRNAs and lncRNAs were found to be associated with ovarian development, with some mRNA and ncRNA functions associated with steroid hormone. In addition, we constructed and visualized the lncRNA/circRNA-miRNA-mRNA network based on ceRNA targeting relationships. These findings may facilitate our further understanding the mRNA and ncRNAs roles in the regulation of shellfish reproduction.
Sections du résumé
BACKGROUND
BACKGROUND
Ovarian development is an important prerequisite and basis for animal reproduction. In many vertebrates, it is regulated by multiple genes and influenced by sex steroid hormones and environmental factors. However, relative information is limited in shellfish. To explore the biological functions and molecular mechanisms of mRNA and non-coding RNA that regulate ovarian development in Scapharca broughtonii, we performed whole transcriptome sequencing analysis on ovaries at three developmental stages. Furthermore, the biological processes involved in the differential expression of mRNA and ncRNA were analyzed.
RESULTS
RESULTS
A total of 11,342 mRNAs, 6897 lncRNAs, 135 circRNAs, and 275 miRNAs were differentially expressed. By mapping the differentially expressed RNAs from the three developmental stages of Venn diagram, multiple groups of shared mRNAs and lncRNAs were found to be associated with ovarian development, with some mRNA and ncRNA functions associated with steroid hormone. In addition, we constructed and visualized the lncRNA/circRNA-miRNA-mRNA network based on ceRNA targeting relationships.
CONCLUSIONS
CONCLUSIONS
These findings may facilitate our further understanding the mRNA and ncRNAs roles in the regulation of shellfish reproduction.
Identifiants
pubmed: 37736709
doi: 10.1186/s12864-023-09648-z
pii: 10.1186/s12864-023-09648-z
pmc: PMC10515027
doi:
Substances chimiques
RNA, Messenger
0
RNA, Long Noncoding
0
RNA, Untranslated
0
MicroRNAs
0
RNA, Circular
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
563Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
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